Tsujino Tetsuhiro, Takahashi Akira, Watanabe Taisuke, Isobe Kazushige, Kitamura Yutaka, Okuda Kazuhiro, Nakata Koh, Kawase Tomoyuki
Tokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, Japan.
Private Practice, Takatsu-ku, Kawasaki 213-0033, Japan.
Dent J (Basel). 2019 Nov 19;7(4):109. doi: 10.3390/dj7040109.
Recent progress in the industrial development of dental implants has improved their surface bio-affinity, while clinical implantologists attempt to improve it through coating with various compounds, including platelet-rich plasma (PRP) in clinical settings. However, it is poorly understood how PRP acts on titanium surfaces. To validate this surface modification method and demonstrate how platelet-derived soluble biomolecules released from the activated adherent platelets act on plain, commercially pure-titanium (-Ti) plates, we evaluated the distribution of biomolecules by immunofluorescence. PPARγ, PDGF-B, and TGFβ1 were similarly released at immunofluorescence levels from activated adherent platelets, retained in the surrounding extra-platelet spaces for a while, and did not immediately diffuse away to distant spaces. Exogenously added CaCl augmented release and retention of those biomolecules along with activation and aggregation. Taken together with our previous data regarding platelet adhesion, these findings suggest that especially when treated with CaCl, platelets immediately adhere on -Ti plates to release their stored biomolecules in the absence of plasma proteins and that these biomolecules do not diffuse away, but stay longer in extra-platelet spaces around the platelets by newly formed, immature fibrin fiber fragments. Consequently, these retained biomolecules are anticipated to cooperatively stabilize implants by stimulating alveolar bone regeneration and integration.
牙科植入物工业发展的最新进展改善了其表面生物亲和力，而临床植入专家试图通过在临床环境中用包括富血小板血浆（PRP）在内的各种化合物进行涂层来进一步提高其表面生物亲和力。然而，人们对PRP如何作用于钛表面了解甚少。为了验证这种表面改性方法，并证明从活化的粘附血小板释放的血小板衍生可溶性生物分子如何作用于普通的商业纯钛（-Ti）板，我们通过免疫荧光评估了生物分子的分布。PPARγ、PDGF-B和TGFβ1从活化的粘附血小板中以相似的免疫荧光水平释放出来，在周围的血小板外空间中保留一段时间，并且不会立即扩散到远处空间。外源添加CaCl会增加这些生物分子的释放和保留，同时促进活化和聚集。结合我们之前关于血小板粘附的数据，这些发现表明，特别是在用CaCl处理时，血小板会立即粘附在-Ti板上，在没有血浆蛋白的情况下释放其储存的生物分子，并且这些生物分子不会扩散，而是通过新形成的、不成熟的纤维蛋白纤维片段在血小板周围的血小板外空间中停留更长时间。因此，这些保留的生物分子有望通过刺激牙槽骨再生和整合来协同稳定植入物。
