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果胶-壳聚糖水凝胶的溶胀、蛋白质吸附及生物相容性

Swelling, Protein Adsorption, and Biocompatibility of Pectin-Chitosan Hydrogels.

作者信息

Popov Sergey, Paderin Nikita, Chistiakova Elizaveta, Sokolova Alisa, Konyshev Ilya V, Belozerov Vladislav S, Byvalov Andrey A

机构信息

Institute of Physiology of Federal Research Centre "Komi Science Centre of the Urals Branch of the Russian Academy of Sciences", 50 Pervomaiskaya Str., 167982 Syktyvkar, Russia.

出版信息

Gels. 2024 Jul 17;10(7):472. doi: 10.3390/gels10070472.

DOI:10.3390/gels10070472
PMID:39057495
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11275652/
Abstract

The study aims to determine how chitosan impacts pectin hydrogel's ability to attach peritoneal leukocytes, activate complement, induce hemolysis, and adsorb blood proteins. The hydrogels PEC-Chi0, PEC-Chi25, PEC-Chi50, and PEC-Chi75 were prepared by placing a mixture solution of 4% pectin and 4% chitosan in a ratio of 4:0, 3:1, 2:2, and 1:3 in a solution of 1.0 M CaCl. Chitosan was found to modify the mechanical properties of pectin-calcium hydrogels, such as hardness and cohesiveness-to-adhesiveness ratio. Chitosan in the pectin-calcium hydrogel caused pH-sensitive swelling in Hanks' solution. The PEC-Chi75 hydrogel was shown to adsorb serum proteins at pH 7.4 to a greater extent than other hydrogels. PEC-Chi75's strong adsorption capacity was related to lower peritoneal leukocyte adherence to its surface when compared to other hydrogels, showing improved biocompatibility. Using the optical tweezers approach, it was shown that the force of interaction between pectin-chitosan hydrogels and plasma proteins increased from 10 to 24 pN with increasing chitosan content from 0 to 75%. Thus, the properties of pectin-calcium hydrogel, which determine interactions with body tissues after implantation, are improved by the addition of chitosan, making pectin-chitosan hydrogel a promising candidate for smart biomaterial development.

摘要

该研究旨在确定壳聚糖如何影响果胶水凝胶附着腹膜白细胞、激活补体、诱导溶血和吸附血液蛋白的能力。通过将4%果胶和4%壳聚糖以4:0、3:1、2:2和1:3的比例混合溶液置于1.0 M氯化钙溶液中，制备了水凝胶PEC-Chi0、PEC-Chi25、PEC-Chi50和PEC-Chi75。发现壳聚糖可改变果胶-钙水凝胶的机械性能，如硬度和内聚性与粘附性之比。果胶-钙水凝胶中的壳聚糖在汉克斯溶液中引起pH敏感的溶胀。结果表明，PEC-Chi75水凝胶在pH 7.4时比其他水凝胶更能吸附血清蛋白。与其他水凝胶相比，PEC-Chi75的强吸附能力与其表面较低的腹膜白细胞粘附有关，显示出改善的生物相容性。使用光镊方法表明，随着壳聚糖含量从0%增加到75%，果胶-壳聚糖水凝胶与血浆蛋白之间的相互作用力从10 pN增加到24 pN。因此，添加壳聚糖可改善果胶-钙水凝胶的性能，这些性能决定了植入后与身体组织的相互作用，使果胶-壳聚糖水凝胶成为智能生物材料开发的有前途的候选材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12a5/11275652/f0421e4e9c30/gels-10-00472-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12a5/11275652/1dbde6b458a1/gels-10-00472-g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12a5/11275652/22eaa675fbad/gels-10-00472-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12a5/11275652/6c1b641a68ba/gels-10-00472-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12a5/11275652/47ea7c3cdff5/gels-10-00472-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12a5/11275652/8aa0a4b274f4/gels-10-00472-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12a5/11275652/5e8d84608f26/gels-10-00472-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12a5/11275652/1dbde6b458a1/gels-10-00472-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12a5/11275652/f0421e4e9c30/gels-10-00472-g010.jpg

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