Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Mater Sci Eng C Mater Biol Appl. 2020 Jan;106:110183. doi: 10.1016/j.msec.2019.110183. Epub 2019 Sep 10.
A sensitive electrochemical sensor for detection of morphine (MPH) at the surface of electrode modified with electrospun magnetic nanofibers (MNFs) was prepared. The features of constructed sensor were evaluated by scanning electron microscopy (SEM), X ray diffraction (XRD) and electrochemical impedance spectroscopy (EIS). The modified sensor was used for MPH analysis using of cyclic voltammetry (CV) and differential pulse voltammetry (DPV) method. The calibration curve has been composed of a linear portion in the concentration range of 0.0033-55 μM and 55-245 μM and the detection limit was 1.9 nM. The reproducibility of the peak current with a reliable relative standard deviation (RSD) value was acquired. Based on the results, the fabricated sensor has good stability and reproducibility, as well as the sensitive and selective analysis of MPH in human serum samples as real samples had effectively been feasible. The results of the actual sample were measured by HPLC procedure, and the results were compared with the results of the electrochemical method and corroborated them.
一种用于在电纺磁性纳米纤维（MNF）修饰电极表面检测吗啡（MPH）的灵敏电化学传感器已经制备。通过扫描电子显微镜（SEM）、X 射线衍射（XRD）和电化学阻抗谱（EIS）评估了所构建传感器的特性。使用循环伏安法（CV）和差分脉冲伏安法（DPV）对修饰后的传感器进行了 MPH 分析。校准曲线在浓度范围为 0.0033-55 μM 和 55-245 μM 时呈现线性部分，检测限为 1.9 nM。获得了具有可靠相对标准偏差（RSD）值的峰电流重现性。基于这些结果，所制备的传感器具有良好的稳定性和重现性，以及对人血清样品中 MPH 的灵敏和选择性分析，实际样品的分析是可行的。通过 HPLC 程序测量了实际样品的结果，并将结果与电化学方法的结果进行了比较和验证。
