综合代谢分析为江华苦茶（江华变种）不同组织中的初级和次级代谢产物提供了新的见解。

Comprehensive metabolic analyses provide new insights into primary and secondary metabolites in different tissues of Jianghua Kucha tea ( var. cv. Jianghua).

作者信息

Wu Wenliang, Shi Jiang, Jin Jiqiang, Liu Zhen, Yuan Yong, Chen Zhida, Zhang Shuguang, Dai Weidong, Lin Zhi

机构信息

Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.

Tea Research Institute, Hunan Academy of Agricultural Sciences, Changsha, Hunan, China.

出版信息

Front Nutr. 2023 May 19;10:1181135. doi: 10.3389/fnut.2023.1181135. eCollection 2023.

DOI:10.3389/fnut.2023.1181135

PMID:37275632

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10235520/

Abstract

BACKGROUND

Jianghua Kucha (JHKC) is a special tea germplasm with enriched specialized secondary metabolites, including theacrine, non-epimeric flavanols and methylated flavanols. Moreover, primary metabolites provide precursors and energy for the production of secondary metabolites. However, the accumulation patterns of primary and secondary metabolites in different tissues of JHKC are unclear.

METHODS

The changes of primary and secondary metabolites and related metabolic pathways (primary and secondary metabolism) in different JHKC tissues (the bud, 1st-4th leaves, and new stem) were investigated via metabolomics analysis with ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS).

RESULTS

Significant differences were observed in 68 primary and 51 secondary metabolites mainly related with the pathways of starch and sucrose, amino acids, caffeine, and flavanols metabolism and TCA cycle. The bud exhibited higher levels of glucose-6-phosphate, citric acid, most amino acids, theobromine, catechin-gallate, epicatechin-gallate, procyanidins, and theasinensins; the 1st leaf showed higher levels of caffeine and epigallocatechin-3-gallate; and the 4th leaf contained higher levels of most monosaccharides, theacrine, and epigallocatechin-3-O-(3"-O-methyl)-gallate. In addition, primary metabolites and important secondary metabolites had certain correlations.

CONCLUSION

This study provides comprehensive insight into primary and secondary metabolites in JHKC and offers guidelines for efficiently utilizing specialized metabolites of JHKC in the future.

摘要

背景

江华苦茶（JHKC）是一种特殊的茶树种质资源，富含多种次生代谢产物，包括茶氨酸、非表异构黄烷醇和甲基化黄烷醇。此外，初级代谢产物为次生代谢产物的合成提供前体和能量。然而，江华苦茶不同组织中初级和次生代谢产物的积累模式尚不清楚。

方法

采用超高效液相色谱四极杆飞行时间质谱（UHPLC-QTOF/MS）代谢组学分析方法，研究江华苦茶不同组织（芽、第1-4叶和新梢）中初级和次生代谢产物及其相关代谢途径（初级和次生代谢）的变化。

结果

在68种初级代谢产物和51种次生代谢产物中观察到显著差异，这些代谢产物主要与淀粉和蔗糖代谢、氨基酸代谢、咖啡因代谢、黄烷醇代谢以及三羧酸循环途径有关。芽中葡萄糖-6-磷酸、柠檬酸、大多数氨基酸、可可碱、儿茶素没食子酸酯、表儿茶素没食子酸酯、原花青素和茶黄素的含量较高；第1叶中咖啡因和表没食子儿茶素-3-没食子酸酯的含量较高；第4叶中大多数单糖、茶氨酸和表没食子儿茶素-3-O-（3”-O-甲基）-没食子酸酯的含量较高。此外，初级代谢产物与重要的次生代谢产物之间存在一定的相关性。