Zhou Shengen, Hu Zongli, Li Fenfen, Tian Shibing, Zhu Zhiguo, Li Anzhou, Chen Guoping
1Laboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing, People's Republic of China.
2Institute of Vegetable Research, Chongqing Academy of Agricultural Sciences, Chongqing, People's Republic of China.
Hortic Res. 2019 Nov 15;6:125. doi: 10.1038/s41438-019-0207-6. eCollection 2019.
The gene was initially identified in tomato and serves as a key regulator of fruit shape. There are 31 OFP members in the tomato genome. However, their roles in tomato growth and reproductive development are largely unknown. Here, we cloned the transcription factor . Tomato plants overexpressing displayed several phenotypic defects, including an altered floral architecture and fruit shape and reduced male fertility. overexpression altered the expression levels of some brassinosteroid (BR)-associated genes, implying that SlOFP20 may play a negative role in the BR response, similar to its ortholog OsOFP19 in rice. Moreover, the transcript accumulation of gibberellin (GA)-related genes was significantly affected in the transgenic lines. SlOFP20 may play an important role in the crosstalk between BR and GA. The pollen germination assay suggested that the pollen germination rate of -OE plants was distinctly lower than that of WT plants. In addition, the tomato pollen-associated genes , , , , and were all suppressed in the transgenic lines. Our data imply that may affect floral organ and pollen development by modulating BR and GA signaling in tomato.
该基因最初在番茄中被鉴定出来，是果实形状的关键调节因子。番茄基因组中有31个OFP成员。然而，它们在番茄生长和生殖发育中的作用在很大程度上尚不清楚。在此，我们克隆了该转录因子。过表达该转录因子的番茄植株表现出多种表型缺陷，包括花结构改变、果实形状改变以及雄性育性降低。过表达改变了一些与油菜素内酯（BR）相关基因的表达水平，这意味着SlOFP20可能在BR反应中起负作用，类似于其在水稻中的直系同源基因OsOFP19。此外，转基因株系中赤霉素（GA）相关基因的转录积累受到显著影响。SlOFP20可能在BR和GA的相互作用中起重要作用。花粉萌发试验表明，过表达植株的花粉萌发率明显低于野生型植株。此外，番茄花粉相关基因、、、和在转基因株系中均受到抑制。我们的数据表明，该转录因子可能通过调节番茄中的BR和GA信号来影响花器官和花粉发育。
