Departament of Genetic, Evolution, Microbiology and Imunology, Institute of Biology, State University of Campinas, Campinas, Brazil.
Curr Microbiol. 2020 Feb;77(2):179-184. doi: 10.1007/s00284-019-01812-7. Epub 2019 Nov 21.
Mobile genetic elements (MGE) play a large role in the plasticity of genomes, participating in several phenomena which involve genes acquisition. Pseudomonas stutzeri is an environmental widely distributed bacteria. This bacteria has a very large genomic plasticity, which would explain its occurrence in several different environments. NCBI data bank and online programs were used to build an inventory to investigate diversity and structure of MGE in Pseudomonas stutzeri, searching for insertion sequences (IS), integrases/transposases, plasmids and prophages. Five hundred and forty-eight ISs, 62 integrases, 166 transposases, five plasmids and eight complete prophages were found. MGE location and adjacent genes were investigated. Possible implications of the presence of these mobile elements explaining phenotypic diversity of Pseudomonas stutzeri were discussed. The study showed that MGEs might be good clues to understand the dynamics of genomes and their phenotypic plasticity, although they are not the only elements responsible for these characteristics.
移动遗传元件（MGE）在基因组的可塑性中起着重要作用，参与了涉及基因获取的几种现象。施氏假单胞菌是一种广泛分布于环境中的细菌。这种细菌具有非常大的基因组可塑性，这可以解释它在几种不同环境中的存在。我们使用 NCBI 数据库和在线程序构建了一个清单，以调查施氏假单胞菌中 MGE 的多样性和结构，搜索插入序列（IS）、整合酶/转座酶、质粒和噬菌体。发现了 548 个 IS、62 个整合酶、166 个转座酶、5 个质粒和 8 个完整的噬菌体。研究了 MGE 的位置及其相邻基因。讨论了这些移动元件的存在对施氏假单胞菌表型多样性的可能影响。该研究表明，MGE 可能是理解基因组动态及其表型可塑性的重要线索，尽管它们不是导致这些特征的唯一因素。
