Lee Chanjae, Bae Min K, Choi Nakjun, Lee Su Jeong, Lee Sung-Jae
Department of Biology, Kyung Hee University, Seoul, Korea.
Department of Geography, Kyung Hee University, Seoul, Korea.
Bioinform Biol Insights. 2021 Aug 4;15:11779322211037437. doi: 10.1177/11779322211037437. eCollection 2021.
The genome of the radiation-resistant bacterium contains 19 types of insertion sequences (ISs), including 93 total transposases (Tpases) in 73 full-length ISs from the main chromosome and 2 mega plasmids. In this study, 68 ISs from the genome were extracted to implicate the earlier genome before its mutation by transposition of ISs. The total size of eliminated ISs from genome was 78.85 kb. From these in silico corrections of mutation by the ISs, we have become aware of some bioinformatics factualness as follows: (1) can reassemble the disrupted genes if the exact IS region was eliminated, (2) can configure the schematic clustering of major DDE type Tpases, (3) can determine IS integration order across multiple hot spots, and (4) can compare genetic relativeness by the partial synteny analysis between and strain S9. Recently, we found that several IS elements actively transferred to other genomic sites under hydrogen peroxide-induced oxidative stress conditions, resulting in the inactivation of functional genes. Therefore, the single species genome's mobilome study provides significant support to define bacterial genome plasticity and molecular evolution from past and present progressive transposition events.
耐辐射细菌的基因组包含19种插入序列（ISs），包括来自主染色体和2个大质粒的73个全长ISs中的93个转座酶（Tpases）。在本研究中，从该基因组中提取了68个ISs，以推断其在通过ISs转座发生突变之前的早期基因组。从基因组中消除的ISs的总大小为78.85 kb。通过这些对ISs突变的计算机模拟校正，我们了解到以下一些生物信息学事实：（1）如果消除了确切的IS区域，可以重新组装被破坏的基因；（2）可以构建主要DDE型Tpases的示意图聚类；（3）可以确定跨多个热点的IS整合顺序；（4）可以通过与菌株S9之间的部分共线性分析比较遗传相关性。最近，我们发现几种IS元件在过氧化氢诱导的氧化应激条件下会主动转移到其他基因组位点，导致功能基因失活。因此，对单一物种基因组的可移动基因组研究为从过去和现在的渐进转座事件定义细菌基因组可塑性和分子进化提供了重要支持。
