College of Science, Health, Engineering and Education, Murdoch University, Murdoch, Western Australia, 6150, Australia.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, Jiangsu, People's Republic of China.
Parasitol Res. 2020 Feb;119(2):611-621. doi: 10.1007/s00436-019-06546-w. Epub 2019 Nov 21.
A new Caryospora-like isolate is described from a magpie-lark (Grallina cyanoleuca) in Western Australia. Sporulated oocysts of the Caryospora-like isolate (n = 35) are subspherical with a shape index of 1.13 ((21.5 (19.7-23.6) × 19.0 (18.1-19.8) μm). The bilayered oocyst wall is smooth. Micropyle, polar granule and oocyst residuum are absent. The sporocyst is ellipsoidal, 18.9 (17.2-20.8) × 12.3 (11.9-12.8) μm, with a shape index (length/width) of 1.54. The sporocyst wall is bilayered. Stieda and substieda bodies are present, the Stieda body is small and flattened and the substieda is trapezoidal. Sporocyst with eight sporozoites arranged head to tail. The sporozoites are vermiform, 18.9 (17.2-20.8) × 12.3 (11.9-12.8) μm and have striations at the anterior end. Each sporozoite has both anterior and posterior refractile bodies. A sporocyst residuum is present. Molecular characterization of the isolated Caryospora-like oocysts was conducted at the 18S ribosomal RNA and the mitochondrial cytochrome oxidase (COI) loci. At the 18S rRNA locus, the Caryospora-like isolate exhibited 88.8% to 96.5% similarity with other Caryospora spp. from different hosts. At the COI locus, it showed 91.5% similarity to Caryospora cf. bigenetica JB-2013 (KF859856) from the rattlesnake, Sistrurus catenatus.
一种新的 Caryospora 样分离株是从西澳大利亚的一种喜鹊 Lark（Grallina cyanoleuca）中描述的。 Caryospora 样分离株的孢子化卵囊（n = 35）为亚球形，形状指数为 1.13（（21.5（19.7-23.6）×19.0（18.1-19.8）μm）。双层卵囊壁光滑。无微口、极粒和卵囊残余物。孢子囊为椭圆形，18.9（17.2-20.8）×12.3（11.9-12.8）μm，形状指数（长度/宽度）为 1.54。孢子囊壁双层。有 Stieda 和 substieda 体，Stieda 体小而扁平，substieda 体为梯形。带有 8 个以头到尾排列的孢子的孢子囊。孢子呈蠕虫状，18.9（17.2-20.8）×12.3（11.9-12.8）μm，前端有条纹。每个孢子都有前、后折射体。存在孢子囊残余物。对分离的 Caryospora 样卵囊进行了 18S 核糖体 RNA 和线粒体细胞色素氧化酶（COI）基因座的分子特征分析。在 18S rRNA 基因座，Caryospora 样分离株与来自不同宿主的其他 Caryospora spp. 显示出 88.8%至 96.5%的相似性。在 COI 基因座，它与响尾蛇， Sistrurus catenatus 的 Caryospora cf. bigenetica JB-2013（KF859856）显示出 91.5%的相似性。
