Center for Biosystems Dynamics Research, RIKEN, Osaka, Japan.
Dev Growth Differ. 2020 Apr;62(3):167-176. doi: 10.1111/dgd.12637. Epub 2019 Nov 21.
Techniques for partitioning cell adhesion are useful tools in biological and medical experiments. However, conventional cell patterning methods require special apparatus, special materials or high-level skills. Therefore, we have developed a new cell patterning methodology which can be easily carried out in biological laboratories. Non-cell adhesive material including hydrogel or gas patterns to restrict cell adhesion on a culture dish or glass substrates can be constructed by exploiting a polydimethylsiloxane (PDMS) mold with microchannels. The PDMS molds suck non-adhesive materials into microchannels from the inlet of the microchannels and the materials are immobilized onto the substrates with a desired pattern. High resolution under a few micrometers and long-term stability can be realized. This method has been used for analysis of stem cells, muscle cells, neuron development and other cells in collaboration with many biological researchers. Several examples to use this technique are introduced in this review.
细胞黏附分区技术在生物和医学实验中是一种非常有用的工具。然而，传统的细胞图案化方法需要特殊的仪器、特殊的材料或高级技能。因此，我们开发了一种新的细胞图案化方法，该方法可以在生物实验室中轻松进行。非细胞黏附材料，包括水凝胶或气体图案，可以通过利用具有微通道的聚二甲基硅氧烷（PDMS）模具来构建，以限制细胞在培养皿或玻璃基板上的黏附。PDMS 模具从微通道的入口将非黏附材料吸入微通道中，然后将材料固定到具有所需图案的基板上。可以实现几微米以下的高分辨率和长期稳定性。该方法已与许多生物研究人员合作，用于分析干细胞、肌肉细胞、神经元发育和其他细胞。本文介绍了该技术的几个应用实例。
