Microfluidics Cluster UPV/EHU, BIOMICs Microfluidics Group, Lascaray Research Center, University of the Basque Country UPV/EHU, Vitoria-Gasteiz, Spain.
BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU, Vitoria-Gasteiz, Spain.
Anal Bioanal Chem. 2024 Dec;416(30):7249-7266. doi: 10.1007/s00216-024-05435-1. Epub 2024 Jul 24.
Monitoring cell secretion in complex microenvironments is crucial for understanding cellular behavior and advancing physiological and pathological research. While traditional cell culture methods, including organoids and spheroids, provide valuable models, real-time monitoring of cell secretion of signaling molecules remains challenging. Integrating advanced monitoring technologies into these systems often disrupts the delicate balance of the microenvironment, making it difficult to achieve sensitivity and specificity. This review explored recent strategies for integrating the monitoring of cell secretion of signaling molecules, crucial for understanding and replicating cell microenvironments, within cell culture platforms, addressing challenges such as non-adherent cell models and the focus on single-cell methodologies. We highlight advancements in biosensors, microfluidics, and three-dimensional culture methods, and discuss their potential to enhance real-time, multiplexed cell monitoring. By examining the advantages, limitations, and future prospects of these technologies, we aim to contribute to the development of integrated systems that facilitate comprehensive cell monitoring, ultimately advancing biological research and pharmaceutical development.
监测复杂微环境中的细胞分泌对于理解细胞行为和推进生理及病理研究至关重要。虽然传统的细胞培养方法，包括类器官和球体，提供了有价值的模型，但对细胞分泌的信号分子进行实时监测仍然具有挑战性。将先进的监测技术整合到这些系统中往往会破坏微环境的微妙平衡，难以实现灵敏度和特异性。本综述探讨了将信号分子细胞分泌监测整合到细胞培养平台中的最新策略，这些策略对于理解和复制细胞微环境至关重要，同时解决了非贴壁细胞模型的挑战以及对单细胞方法的关注。我们重点介绍了生物传感器、微流控和三维培养方法的进展，并讨论了它们在增强实时、多重细胞监测方面的潜力。通过检查这些技术的优势、限制和未来前景，我们旨在为开发促进全面细胞监测的综合系统做出贡献，从而最终推进生物研究和药物开发。
