Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Oil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Key Laboratory of Oilseeds Processing, Ministry of Agriculture and Rural Affairs, No. 2 Xudong 2nd Road, Wuhan 430062, China.
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Oil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Key Laboratory of Oilseeds Processing, Ministry of Agriculture and Rural Affairs, No. 2 Xudong 2nd Road, Wuhan 430062, China.
Int J Biol Macromol. 2020 Jun 15;153:1107-1116. doi: 10.1016/j.ijbiomac.2019.10.241. Epub 2019 Nov 19.
Flaxseed oligosaccharides (FGOS) were prepared by degradation of flaxseed gum (FG) using enzymatic method. Factors affecting the enzymatic hydrolysis of FG were investigated by single factor and orthogonal tests. In the optimum hydrolysis conditions (reaction time 12 h, temperature 50 °C, pH 4.5, cellulase concentration 100 U/mL), the reducing sugar ratio and extraction yield of FGOS were 33.6 ± 0.35% and 56.8 ± 0.41%, respectively. The average molecular weight of FGOS was about 1.6 kDa, which consists of mannose, galactose, glucose, arabinose, glucuronic acid, xylose, rhamnose, ribose, galacturonic acid. Fourier-transform infrared spectra and NMR indicated that FG was successfully degraded to FGOS. FGOS exhibited better antioxidant activities than FG on scavenging hydroxyl, ABTS and DPPH radicals. In vitro cytotoxicities experiments reveal FGOS acquire the ability of antiproliferation against HepG2 and Hela cells in a dose-dependent manner.
亚麻籽低聚糖（FGOS）是通过酶解法降解亚麻籽胶（FG）制备的。通过单因素和正交试验研究了影响 FG 酶解的因素。在最佳水解条件（反应时间 12 h、温度 50°C、pH4.5、纤维素酶浓度 100 U/mL）下，FGOS 的还原糖比例和提取率分别为 33.6±0.35%和 56.8±0.41%。FGOS 的平均分子量约为 1.6 kDa，由甘露糖、半乳糖、葡萄糖、阿拉伯糖、葡萄糖醛酸、木糖、鼠李糖、核糖、半乳糖醛酸组成。傅里叶变换红外光谱和 NMR 表明 FG 成功降解为 FGOS。FGOS 在清除羟基、ABTS 和 DPPH 自由基方面的抗氧化活性均优于 FG。体外细胞毒性实验表明，FGOS 对 HepG2 和 Hela 细胞的增殖具有剂量依赖性抑制作用。
