Herrera Hector, Soto Javiera, de Bashan Luz E, Sampedro Inmaculada, Arriagada Cesar
Laboratorio de Biorremediación, Facultad de Ciencias Agropecuarias y Forestales, Departamento de Ciencias Forestales, Universidad de La Frontera, Temuco 4780000, Chile.
The Bashan Institute of Science, 1730 Post Oak Court, Auburn, AL 36830, USA.
Microorganisms. 2019 Nov 20;7(12):586. doi: 10.3390/microorganisms7120586.
The microbiological interactions of the roots of non-photosynthetic plants in South America have been scarcely explored. This study analyzes culturable fungal diversity associated with the mycoheterotrophic plant Phil. (Corsiaceae) in southern Chile, growing in two different understoreys of native (-dominated) and mixed forest (native, and ). Rhizospheric and endophytic fungi were isolated, cultured, and purified to identify microorganisms associated with roots. We showed the different fungi associated with the plant, and that these distributions are influenced by the sampling site. We isolated 410 fungal strains (144 endophytic and 266 from the rhizosphere). We identified 13 operative taxonomical units from plants sampled in the mixed forest, while 15 were from the native forest. Rhizospheric microorganisms were mainly related to spp., whereas some pathogenic and saprophytic strains were more frequent inside the roots. Our results have also shown that the fungal strains are weak for phosphate solubilization, but other pathways such as organic acid exudation and indole acetic acid production can be considered as major mechanisms to stimulate plant growth. Our results point to new fungal associates of plants reported in Andean ecosystems, identifying new beneficial endophytic fungi associated with roots of this fully mycoheterotrophic plant.
南美洲非光合植物根系的微生物相互作用鲜有研究。本研究分析了智利南部与菌根异养植物Phil.（腐生花科）相关的可培养真菌多样性，该植物生长在原生林（以原生植物为主）和混交林（原生植物、[此处原文缺失部分内容]）的两种不同林下植被中。分离、培养和纯化根际和内生真菌，以鉴定与根系相关的微生物。我们展示了与该植物相关的不同真菌，且这些分布受采样地点的影响。我们分离出410株真菌菌株（144株内生真菌和266株根际真菌）。我们从混交林中采样的植物中鉴定出13个操作分类单元，而从原生林中采样的植物中鉴定出15个。根际微生物主要与[此处原文缺失部分内容]属有关，而一些致病和腐生菌株在根内更为常见。我们的结果还表明，真菌菌株溶解磷酸盐的能力较弱，但其他途径如有机酸分泌和吲哚乙酸产生可被视为刺激植物生长的主要机制。我们的结果指出了安第斯生态系统中报道的Phil.植物的新真菌伴生种，鉴定出了与这种完全菌根异养植物根系相关的新的有益内生真菌。
