Department of Biology, Waseda University, 2-2 Wakamatsucho Shinjuku, Tokyo, 162-8480, Japan.
Waseda Research Institute for Science and Engineering, Waseda University, 3-4-1 Okubo Shinjuku, Tokyo, 169-8555, Japan.
Anal Sci. 2021 Oct 10;37(10):1469-1472. doi: 10.2116/analsci.21N009. Epub 2021 Mar 19.
An enzyme immunoassay, in which an enzyme (e.g., alkaline phosphatase, ALP) is conjugated with an antibody, is a precise and simple protein detection method. Precise measurements of enzymes at low concentrations allow for ultrasensitive protein detection. The application of a phosphorylated substrate to ALP, followed by using a dephosphorylated substrate in thionicotinamide-adenine dinucleotide cycling, provides a simple and precise quantification of ALP. We describe a protocol for detecting ALP at the zeptomole level using a simple colorimetric method.
酶免疫分析，即将酶（如碱性磷酸酶，ALP）与抗体结合，是一种精确而简单的蛋白质检测方法。对低浓度酶的精确测量可实现对蛋白质的超高灵敏度检测。将磷酸化底物应用于 ALP，然后在硫代烟酰胺腺嘌呤二核苷酸循环中使用去磷酸化底物，可简单而精确地定量 ALP。我们描述了一种使用简单比色法检测皮摩尔级 ALP 的方案。
