Zhang Xiao-Qing, Yao Chang, Bian Wei-He, Chen Xu, Xue Jing-Xian, Zhu Zhi-Yuan, Ying Yu, Xu Yan Lei, Wang Cong
The Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing China.
Food Sci Nutr. 2019 Sep 18;7(11):3403-3413. doi: 10.1002/fsn3.1154. eCollection 2019 Nov.
Traditional Chinese medicine (TCM) has from ancient times been applied in China for the treatment of breast cancer with its own unique theoretical system. Sanhuang decoction composed of astragalus membranaceus, prepared rhubarb, and rhizoma curcumae longae has traditionally been used for antioxidant stress, inflammatory reaction, and angiogenesis. However, the role and mechanism of Sanhuang decoction in breast cancer remains unknown. The present study demonstrated the antitumor activity of Sanhuang decoction against breast cancer xenografts in nude mice. Notably, Sanhuang decoction promoted severe necrosis and induced cell death. In addition, Sanhuang decoction obviously regulated the inflammation and oxidative stress. Despite these, Sanhuang decoction could increase the expression of Nrf2. Moreover, si-Nrf2 exhibited the opposite effects compared with the Sanhuang decoction treatment group and reversed the antibreast cancer role of Sanhuang decoction. Further, Sanhuang decoction remarkably suppressed the expression of PI3K/AKT/mTOR signaling pathway. Taken together, Sanhuang decoction was firstly evaluated to possess potent antibreast cancer effect in vivo through regulation of inflammation and oxidative stress accomplished by up-regulation of Nrf2 via PI3K/AKT/mTOR signaling pathway and Sanhuang decoction might be a powerful candidate formula for antibreast cancer.
中医（TCM）自古以来就在中国用于治疗乳腺癌，有着自己独特的理论体系。由黄芪、制大黄和姜黄组成的三黄汤传统上用于抗氧化应激、炎症反应和血管生成。然而，三黄汤在乳腺癌中的作用和机制仍不清楚。本研究证明了三黄汤对裸鼠乳腺癌异种移植瘤的抗肿瘤活性。值得注意的是，三黄汤促进了严重坏死并诱导细胞死亡。此外，三黄汤明显调节炎症和氧化应激。尽管如此，三黄汤可增加Nrf2的表达。此外，与三黄汤治疗组相比，si-Nrf2表现出相反的作用，并逆转了三黄汤的抗乳腺癌作用。此外，三黄汤显著抑制PI3K/AKT/mTOR信号通路的表达。综上所述，首次评估三黄汤通过PI3K/AKT/mTOR信号通路上调Nrf2来调节炎症和氧化应激，从而在体内具有强大的抗乳腺癌作用，三黄汤可能是一种强大的抗乳腺癌候选方剂。
