Jafarinezhad Zahra, Rafati Ali, Ketabchi Farzaneh, Noorafshan Ali, Karbalay-Doust Saied
Histomorphometry and Stereology Research Center Shiraz University of Medical Sciences Shiraz Iran.
Department of Physiology Shiraz University of Medical Sciences Shiraz Iran.
Food Sci Nutr. 2019 Sep 10;7(11):3581-3588. doi: 10.1002/fsn3.1210. eCollection 2019 Nov.
Doxorubicin (DOX) is a cardiotoxic drug. To reduce the harmful effects of DOX, two plant-derived components, including curcumin (CUR) and carvacrol (CAR), were considered. This study aimed to assess the protective effects of CUR and CAR on DOX-induced cardiotoxicity using physiological and stereological evaluations. Male rats were randomly allocated to six groups. Group's I-VI received phosphate-buffered saline (PBS), CUR (100 mg kg day), CAR (50 mg kg day), DOX (4 mg kg week), DOX-CUR, and DOX-CAR, respectively. On day 24, plasma troponin I and ECG were analyzed and the left ventricle underwent stereological assessment. The results showed a fivefold increase in troponin I in the DOX-treated animals compared to the PBS ones. Additionally, heart rate and QRS amplitude, respectively, reduced by 18% and 31% and QT interval and QRS duration, respectively, increased by 41% and 24% in the DOX group in comparison with the PBS rats ( < .05). The total volume of the myocardium and vessels and the number of cardiomyocyte nuclei also, respectively, decreased by 30%, 45%, and 43% in the DOX group compared to the PBS animals (atrophy of the ventricular tissues, < .01). Besides, the mean volumes of the connective tissue and cardiomyocytes, respectively, increased by 46% and 52% in the DOX group ( < .01). In the DOX-CUR and DOX-CAR groups, the changes were prevented extensively in comparison with the DOX group ( < .01). Co-administration of CUR or CAR and doxorubicin for 24 days could improve the heart function and structural changes.
阿霉素（DOX）是一种具有心脏毒性的药物。为降低DOX的有害影响，研究考虑了两种植物来源的成分，即姜黄素（CUR）和香芹酚（CAR）。本研究旨在通过生理学和体视学评估来评价CUR和CAR对DOX诱导的心脏毒性的保护作用。雄性大鼠被随机分为六组。第一至六组分别接受磷酸盐缓冲盐水（PBS）、CUR（100毫克/千克/天）、CAR（50毫克/千克/天）、DOX（4毫克/千克/周）、DOX-CUR和DOX-CAR。在第24天，分析血浆肌钙蛋白I和心电图，并对左心室进行体视学评估。结果显示，与PBS组相比，DOX处理组动物的肌钙蛋白I增加了五倍。此外，与PBS组大鼠相比，DOX组的心率和QRS波幅分别降低了18%和31%，QT间期和QRS时限分别增加了41%和24%（P<0.05）。与PBS组动物相比，DOX组心肌和血管的总体积以及心肌细胞核数量也分别减少了30%、45%和43%（心室组织萎缩，P<0.01）。此外，DOX组结缔组织和心肌细胞的平均体积分别增加了46%和52%（P<0.01）。与DOX组相比，DOX-CUR组和DOX-CAR组的这些变化得到了广泛预防（P<0.01）。CUR或CAR与阿霉素联合给药24天可改善心脏功能和结构变化。
