Vogel P, Petterson D S, Berry P H, Frahn J L, Anderton N, Cockrum P A, Edgar J A, Jago M V, Lanigan G W, Payne A L, Culvenor C C
Aust J Exp Biol Med Sci. 1981 Aug;59(4):455-67. doi: 10.1038/icb.1981.39.
A group of highly toxic compounds was isolated from galled seedheads of annual ryegrass (Lolium rigidum Gaud.) containing Corynebacterium rathayi. Purified extracts were resolved by reverse-phase high-performance liquid chromatography into eight main fractions which have been partially characterised and shown to be toxic to nursling rats. A mixture of the toxins also produced clinical signs and brain lesions in lambs consistent with annual ryegrass toxicity. The name 'corynetoxin' is tentatively proposed for the series, individual members being designated according to their order of elution from the high performance liquid chromatography column as corynetoxins 1 to 8. The two main fractions are corynetoxins 3 and 4 of which the former has been crystallised. They appear to be of glycolipid character, 3-hydroxyheptadecanoic acid and a C6 amino sugar being identified among the hydrolysis products of corynetoxin 3, and heptadec-2-enoic acid and a C6 amino sugar from corynetoxin 4.
从感染了拉氏棒状杆菌（Corynebacterium rathayi）的一年生黑麦草（Lolium rigidum Gaud.）虫瘿种子头中分离出一组剧毒化合物。纯化提取物通过反相高效液相色谱法分离为八个主要组分，这些组分已得到部分表征，并显示对乳鼠有毒。毒素混合物在羔羊身上也产生了与一年生黑麦草中毒一致的临床症状和脑部病变。暂定将该系列毒素命名为“棒状杆菌毒素”，各个成员根据其从高效液相色谱柱上洗脱的顺序分别命名为棒状杆菌毒素1至8。两个主要组分是棒状杆菌毒素3和4，前者已结晶。它们似乎具有糖脂特性，在棒状杆菌毒素3的水解产物中鉴定出3-羟基十七烷酸和一种C6氨基糖，在棒状杆菌毒素4的水解产物中鉴定出十七碳-2-烯酸和一种C6氨基糖。
