Humboldt University Berlin, Department of Chemistry, Brook-Taylor-Str. 2, D-12489 Berlin, Germany.
Chem Commun (Camb). 2019 Dec 5;55(98):14817-14820. doi: 10.1039/c9cc06989e.
Unambiguous imaging of C → U edited mRNA calls for a method that distinguishes a locally high concentration of unbound probe or single nucleotide mismatched target from a locally low concentration of matched mRNA target. To address this issue, we combine FIT probes as a "chemical" detection system with the "biological" MS2 technique. Ratio measurements provide a convenient parameter to discriminate the edited from the unedited state of mRNA encoding for GlyR α2 in HEK cells.
明确显示 C→U 编辑的 mRNA 需要一种方法来区分局部高浓度未结合探针或单核苷酸错配靶标与局部低浓度匹配的 mRNA 靶标。为了解决这个问题，我们将 FIT 探针与“生物”MS2 技术结合作为“化学”检测系统。比率测量提供了一个方便的参数来区分 HEK 细胞中编码 GlyR α2 的 mRNA 的编辑和未编辑状态。
