Department of Oral and Maxillofacial Surgery, University Hospital Erlangen, Glueckstraße, Erlangen, Germany.
Department of Materials Science and Engineering (Glass and Ceramics), University of Erlangen-Nuremberg, Martensstraße, Erlangen, Germany.
J Biomed Mater Res B Appl Biomater. 2020 May;108(4):1687-1696. doi: 10.1002/jbm.b.34514. Epub 2019 Nov 25.
The use of nanoscale surface modifications offers a possibility to regulate the bacterial adherence behavior. The aim of this study was to evaluate the influence of nanoporous anodic aluminum oxide of different pore diameters on the bacterial species Streptococcus mitis and Streptococcus mutans. Nanoporous anodic aluminum oxide (AAO) surfaces with an average pore diameter of 15 and 40 nm, polished pure titanium and compact aluminum oxide (alumina) samples as reference material were investigated. S. mitis and mutans were evaluated for initial adhesion and viability after an incubation period of 30 and 120 min. After 30 min a significantly reduced growth of S. mitis and mutans on 15 nm samples compared to specimens with 40 nm pore diameter, alumina and titanium surfaces could be observed (p < .001). Even after 120 min incubation there was a significant difference between the surfaces with 15 nm pore diameter and the remaining samples (p < .001). AAO surfaces with a small pore diameter have an inhibitory effect on the initial adhesion of S. mitis and mutans. The use of such pore dimensions in the area of the implant shoulder represents a possibility to reduce the adhesion behavior of these bacterial species.
纳米级表面修饰的应用提供了一种调控细菌黏附行为的可能性。本研究旨在评估不同孔径纳米多孔阳极氧化铝对细菌物种变形链球菌和远缘链球菌的影响。研究了平均孔径为 15nm 和 40nm 的纳米多孔阳极氧化铝（AAO）表面、抛光纯钛和致密氧化铝（氧化铝）样品作为参考材料。分别在 30min 和 120min 的孵育期后，评估了 S. mitis 和 mutans 的初始黏附和活力。在 30min 时，与 40nm 孔径、氧化铝和钛表面的样本相比，15nm 样本中 S. mitis 和 mutans 的生长明显减少（p<.001）。即使在 120min 孵育后，15nm 孔径的表面与其余样本之间仍存在显著差异（p<.001）。具有小孔径的 AAO 表面对 S. mitis 和 mutans 的初始黏附具有抑制作用。在种植体肩台区域使用这种孔径尺寸为减少这些细菌物种的黏附行为提供了一种可能性。
