Narendrakumar Krunal, Kulkarni Mukta, Addison Owen, Mazare Anca, Junkar Ita, Schmuki Patrik, Sammons Rachel, Iglič Aleš
School of Dentistry, University of Birmingham, St Chad's Queensway, Birmingham B4 6NN, UK.
Laboratory of Biophysics, Faculty of Electrical Engineering, University of Ljubljana, Ljubljana SI-1000, Slovenia.
Dent Mater. 2015 Dec;31(12):1460-8. doi: 10.1016/j.dental.2015.09.011. Epub 2015 Oct 23.
Peri-implantitis and peri-mucositis pose a severe threat to the success of dental implants. Current research focuses on the development of surfaces that inhibit biofilm formation while not inferring with tissue integration. This study compared the adherence of two oral bacterial species, Streptococcus sanguinis and Streptococcus mutans to nanostructured titanium surfaces.
The samples included TiO2 nanotubes formed by anodization of titanium foil of 100, 50 and 15nm diameter (NT15, NT50, NT100), a nanoporous (15nm pore diameter) surface and compact TiO2 control. Adherent surviving bacteria were enumerated after 1h in an artificial saliva medium containing bovine mucin.
Lowest numbers of adherent bacteria of both species were recovered from the original titanium foil and nanoporous surface and highest numbers from the Ti100 nanotubes. Numbers of attached S. sanguinis increased in the order (NT15<NT50<NT100), correlated with increasing percentage of surface fluoride. The lowest adhesion of S. sanguinis and S. mutans on TiO2 nanostructured surfaces was observed for small diameter nanoporous surfaces which coincides with the highest osteoblast adhesion on small diameter nanotubular/nanoporous surfaces shown in previous work.
This study indicates that the adherence of oral streptococci can be modified by titanium anodization and nanotube diameter.
种植体周炎和种植体周围黏膜炎对牙种植体的成功构成严重威胁。目前的研究重点是开发既能抑制生物膜形成又不影响组织整合的表面。本研究比较了两种口腔细菌——血链球菌和变形链球菌在纳米结构钛表面的黏附情况。
样本包括通过对直径为100、50和15nm的钛箔进行阳极氧化形成的TiO₂纳米管（NT15、NT50、NT100）、纳米多孔（孔径15nm）表面和致密TiO₂对照。在含有牛黏蛋白的人工唾液培养基中培养1小时后，对黏附存活的细菌进行计数。
两种细菌黏附数量最少的是原始钛箔和纳米多孔表面，而Ti100纳米管上的黏附数量最多。血链球菌的附着数量按（NT15＜NT50＜NT100）的顺序增加，这与表面氟化物百分比的增加相关。在小直径纳米多孔表面观察到血链球菌和变形链球菌在TiO₂纳米结构表面的黏附力最低，这与先前工作中显示的小直径纳米管/纳米多孔表面上成骨细胞的最高黏附力一致。
本研究表明，钛的阳极氧化和纳米管直径可改变口腔链球菌的黏附情况。
