Kharitonenkov I G, Mukazhanova G N
Vopr Virusol. 1982 Mar-Apr;27(2):181-4.
A two-step method for isolation of HA2 polypeptide, the light chain of influenza virus hemagglutinin, from influenza virions is described. The method consists in obtaining a preparation of surface glycoproteins by means of their solubilization from virions with a cation detergent (cetyl-trimethyl-ammonium bromide) followed by fractionation of this preparation by preparative electrophoresis. Relative molecular weights of HA2 and other polypeptides comprising the virion have been determined. The method was used with a recombinant MRC-11 strain.
本文描述了一种从流感病毒粒子中分离流感病毒血凝素轻链HA2多肽的两步法。该方法包括通过用阳离子去污剂（十六烷基三甲基溴化铵）从病毒粒子中溶解表面糖蛋白来获得制剂，然后通过制备电泳对该制剂进行分级分离。已经测定了HA2和构成病毒粒子的其他多肽的相对分子量。该方法用于重组MRC - 11菌株。
