Ernst B
Institut für Klinische Chemie und Laboratoriumsdiagnostik, Bereiches Medizin der Wilhelm-Pieck-Universität Rostock.
Z Exp Chir Transplant Kunstliche Organe. 1988;21(4):194-8.
We prepared adsorption immobilized Urease (E.C. 3.5.1.5) with a special method of polymer coating of polystyrene granulate as a matrix, and by subsequent adsorption of two different urease products (AWD Dresden, DDR, 3 U/mg, and Serva, Heidelberg, BRD, 41 U/mg). We obtained the strongest activity (4.0 mumol.g-1.min-1) with immobilisates on polystyrene in comparison with polyvinylchloride, and polyurethane layers, if we used AWD urease (PS, PVC, PUR). By using Serva urease we obtained products with a manyfold of activity (12.8 mumol.g.min-1). This high activity enables us to utilize adsorption immobilized urease for the detoxification of biological fluids.
我们采用一种特殊方法,以聚苯乙烯颗粒为基质进行聚合物涂层,随后吸附两种不同的脲酶产品(民主德国德累斯顿的 AWD,3 U/mg,以及联邦德国海德堡的 Serva,41 U/mg),制备了吸附固定化脲酶(E.C. 3.5.1.5)。与聚氯乙烯和聚氨酯层相比,如果使用 AWD 脲酶(PS、PVC、PUR),我们在聚苯乙烯上的固定化产物获得了最强活性(4.0 μmol·g⁻¹·min⁻¹)。通过使用 Serva 脲酶,我们获得了活性高出许多倍的产品(12.8 μmol·g·min⁻¹)。这种高活性使我们能够将吸附固定化脲酶用于生物流体的解毒。