International Research Centre for Nano Handing and Manufacturing of China (CNM), Changchun University of Science and Technology, Changchun 130022, People's Republic of China.
Nanotechnology. 2020 Mar 13;31(11):115101. doi: 10.1088/1361-6528/ab5b35. Epub 2019 Nov 25.
Disulfiram (DSF), an FDA approved drug for the treatment of alcoholism, has shown its effectiveness against diverse cancer types. Thus, we developed a disulfiram-loaded scaffold using the electrospinning method to enhance the stability of DSF and to facilitate its appropriate distribution to tumor tissues. The drug release profile of the disulfiram-loaded scaffold was examined by high-performance liquid chromatography. We obtained mechanical and morphological characterizations of A549 cells treated with different scaffolds by various techniques to evaluate its antitumor properties. This work revealed that the cells after the treatment with the disulfiram-loaded scaffold exhibited a lower height and a larger elastic modulus compared with the untreated cells and those treated with the neat electrospun fibers. The changes were the indicators of cell apoptosis. Taken collectively, the results indicate that DSF was successfully incorporated into the electrospun fibers, and the disulfiram-loaded scaffold has great potential for inhibiting the regional recurrence of cancer.
双硫仑（DSF）是一种经美国食品药品监督管理局批准用于治疗酗酒的药物，已被证明对多种癌症类型有效。因此，我们采用静电纺丝法制备了负载双硫仑的支架，以提高 DSF 的稳定性并促进其向肿瘤组织的适当分布。采用高效液相色谱法考察了载双硫仑支架的药物释放曲线。采用多种技术对不同支架处理的 A549 细胞进行了机械和形态学表征，以评估其抗肿瘤特性。结果表明，与未经处理的细胞和单纯静电纺丝纤维处理的细胞相比，用载双硫仑支架处理的细胞高度降低，弹性模量增大。这些变化是细胞凋亡的指标。综上所述，结果表明 DSF 已成功掺入静电纺丝纤维中，载双硫仑支架在抑制癌症局部复发方面具有很大的潜力。
