Quan Nguyen Van, Thien Dam Duy, Khanh Tran Dang, Tran Hoang-Dung, Xuan Tran Dang
Graduate School for International Development and Cooperation, Hiroshima University, Hiroshima 739-8529, Japan.
Dai Nam Manufacturing & Trade Co. Ltd., 7th District, Ngo Duc Ke Street No 57, Vung Tau City 78212, Vietnam.
Foods. 2019 Nov 21;8(12):602. doi: 10.3390/foods8120602.
We previously reported the inhibitory potentials of momilactones A (MA) and B (MB) against key enzymes related to type 2 diabetes and obesity. In this study, antioxidant and anti-skin-aging activities of MA and MB were investigated and compared with tricin, a well-known antioxidant and antiaging flavonoid in rice. MA, MB, and tricin were purified from rice husk by column chromatography and their biological activities were subsequently assayed by in vitro trials. The contents of MA, MB, and tricin of different commercial rice cultivars in Japan were quantified and confirmed by ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS) and high-performance liquid chromatography (HPLC) analyses. The antioxidant assays revealed a synergistic activity of the mixture MA and MB (MAB, 1:1, ). In addition, in 2,2'-azino-bis (ABTS) assay, IC values of MAB (0.3 mg/mL) and tricin (0.3 mg/mL) was 4-fold and 9-fold greater than that of individual MB (1.3 mg/mL) or MA (2.8 mg/mL), respectively. The in vitro enzymatic assays on pancreatic elastase and tyrosinase indicated that MA and MB were potential to relief skin wrinkles and freckles. In detail, MA exerted higher inhibition on both enzymatic activities (30.9 and 37.6% for elastase and tyrosinase inhibition, respectively) than MB (18.5 and 12.6%) and MAB (32.0 and 19.7%) at a concentration of 2.0 mg/mL. Notably, MA and the mixture MAB exhibited stronger inhibitions on elastase and tyrosinase in comparison with tricin and vanillin. MA, MB, and tricin in rice are potential to develop cosmetics as well as supplements for skin aging treatments.
我们之前报道了稻壳醇A（MA）和稻壳醇B（MB）对与2型糖尿病和肥胖相关的关键酶的抑制作用。在本研究中，对MA和MB的抗氧化及抗皮肤衰老活性进行了研究，并与水稻中一种著名的抗氧化和抗老化类黄酮小麦黄素进行了比较。通过柱色谱法从稻壳中纯化出MA、MB和小麦黄素，随后通过体外试验测定它们的生物活性。采用超高效液相色谱-电喷雾电离-质谱联用仪（UPLC-ESI-MS）和高效液相色谱（HPLC）分析对日本不同商业水稻品种中MA、MB和小麦黄素的含量进行了定量和确认。抗氧化试验显示MA和MB的混合物（MAB，1:1）具有协同活性。此外，在2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）试验中，MAB（0.3 mg/mL）和小麦黄素（0.3 mg/mL）的半数抑制浓度（IC）分别比单独的MB（1.3 mg/mL）或MA（2.8 mg/mL）高4倍和9倍。对胰弹性蛋白酶和酪氨酸酶的体外酶活性试验表明，MA和MB具有减轻皮肤皱纹和雀斑的潜力。具体而言，在浓度为2.0 mg/mL时，MA对两种酶活性的抑制作用（弹性蛋白酶和酪氨酸酶抑制率分别为30.9%和37.6%）高于MB（18.5%和12.6%）和MAB（32.0%和19.7%）。值得注意的是，与小麦黄素和香草醛相比，MA和混合物MAB对弹性蛋白酶和酪氨酸酶的抑制作用更强。水稻中的MA、MB和小麦黄素具有开发化妆品以及用于皮肤衰老治疗补充剂的潜力。
