Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
Department of Oral and Maxillofacial Surgery, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
J Biomed Mater Res B Appl Biomater. 2020 May;108(4):1669-1678. doi: 10.1002/jbm.b.34512. Epub 2019 Nov 26.
Calcium phosphate cement (CPC) is widely used as a repair material for bone defects. However, there is no report on the use of biphasic CPC combined with dental pulp stem cells (DPSCs) for bone tissue engineering. The aim of this study was to construct biphasic CPC and to investigate its effect in the osteogenic stimulation of human DPSCs (hDPSCs) and macrophage polarization. Biphasic CPC was developed by adding different ratios of β-TCP to an equimolar mixture of tetracalcium phosphate (TTCP) and dicalcium phosphate anhydrous (DCPA). The biocompatibility, osteogenic capacity, and inflammatory reactions of the cements were examined. Our results show that biphasic CPC containing both HA and β-TCP was successfully fabricated. The addition of β-TCP did not increase necrosis or apoptosis of DPSCs. Significant increases in hDPSC mineral deposition and osteogenic marker (ColI and ALP) expression were observed in 20% TCP-CPC. The addition of β-TCP did not promote macrophage polarization to a proinflammatory phenotype. In conclusion, biphasic CPC with hDPSCs for bone regeneration was developed for the first time; the biphasic CPC combined with hDPSCs is expected to be used for the repair and regeneration of bone.
磷酸钙骨水泥（CPC）被广泛用作修复骨缺损的材料。然而，目前尚无关于双相 CPC 与牙髓干细胞（DPSCs）联合用于骨组织工程的报道。本研究旨在构建双相 CPC，并研究其对人牙髓干细胞（hDPSCs）和成骨刺激和巨噬细胞极化的影响。通过向等摩尔混合的磷酸四钙（TTCP）和无水磷酸二钙（DCPA）中添加不同比例的 β-TCP 来开发双相 CPC。对水泥的生物相容性、成骨能力和炎症反应进行了研究。我们的结果表明，成功制备了含有 HA 和 β-TCP 的双相 CPC。β-TCP 的添加不会增加 DPSCs 的坏死或凋亡。在 20%TCP-CPC 中观察到 hDPSC 矿化沉积和成骨标志物（ColI 和 ALP）表达显著增加。β-TCP 的添加不会促进巨噬细胞向促炎表型极化。总之，首次开发了用于骨再生的含 hDPSCs 的双相 CPC；预计双相 CPC 与 hDPSCs 联合用于骨的修复和再生。
