Yamada T, Hirai Y, Sakano S, Kosaka M, Tada K, Furutani S, Kosaka F
Department of Anesthesiology and Resuscitology, Okayama University Medical School, Japan.
Acta Med Okayama. 1988 Aug;42(4):183-92. doi: 10.18926/AMO/30998.
The direct determination by gas chromatography of blood levels of anesthetic agents has been difficult because of the water content of blood. In the present study, the method of Yokota et al. (1967) was modified by improving the packing materials of the column, the blood sample vaporizer and the flow-path during analysis. As a result, accurate and reproducible determination of halothane, enflurane and isoflurane dissolved in blood was achieved. With this system, blood in which halothane, enflurane and isoflurane had been dissolved could be analyzed without changing the column between samples. Moreover, each sample was prepared in less than 10 min, and more than 100 consecutive determinations could be made with excellent reproducibility. The coefficient of variation was less than 3.8%.
由于血液中的水分，通过气相色谱法直接测定血液中麻醉剂的含量一直很困难。在本研究中，对横田等人（1967年）的方法进行了改进，改进了分析过程中柱的填充材料、血样蒸发器和流路。结果，实现了对溶解在血液中的氟烷、恩氟烷和异氟烷的准确且可重复的测定。使用该系统，溶解有氟烷、恩氟烷和异氟烷的血液在不更换柱的情况下即可进行分析。此外，每个样品的制备时间不到10分钟，并且可以进行100多次连续测定，重现性极佳。变异系数小于3.8%。
