College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China; Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Central South University, Changsha, 410083, China.
Chemosphere. 2020 Mar;243:125398. doi: 10.1016/j.chemosphere.2019.125398. Epub 2019 Nov 19.
Different ionomic profiles of plants are associated with different external stresses to which they are exposed. Investigation of ionomic variation is necessary for understanding the migration and detoxification of toxic elements in plants. In the current study, rice plants were treated with arsenite, arsenate, monomethylarsonic acid and dimethylarsinic acid in hydroponics. The ionomic responses of the rice plants to different arsenic (As) species stresses were measured and analyzed. The multielement approach is more sensitive at detecting significant variations from external environmental stresses than the consideration of several individual elements. The pairs of significant correlations between elements varied based on the rice tissues and As species used in treatment, resulting in specific correlation networks. However, some pairs of correlations existed regardless of As species treatment used in this study. Positive correlations between P and Fe were observed in rice roots treated with any of the As species, implying that P and Fe share similar biological processes. The heatmap from hierarchical cluster analysis (HCA) agreed with the principal component analysis (PCA) results in ionomic differentiation between roots and shoots. Furthermore, ionomic differences between rice plants treated with different As species were identified through PCA. This study revealed that the ionomic profiles in rice plants are sufficient to detect responses to environmental perturbations. Association studies between ionomics and genomics are necessary to further understand the potential mechanisms that promote uptake or exclusion of elements in plants.
不同的离子组特征与植物所面临的不同外部胁迫有关。离子组变异的研究对于了解植物中有毒元素的迁移和解毒至关重要。在本研究中，采用水培法在水稻植株上施加亚砷酸盐、砷酸盐、一甲基砷酸和二甲基砷酸。测量和分析了水稻植株对不同砷（As）物种胁迫的离子组响应。与考虑几个个别元素相比，多元素方法更能敏感地检测到来自外部环境胁迫的显著变化。基于处理中使用的水稻组织和 As 物种，元素之间的显著相关对发生变化，从而产生特定的相关网络。然而，无论本研究中使用何种 As 物种处理，都存在一些相关对。在任何 As 物种处理的水稻根中都观察到 P 和 Fe 之间的正相关，这表明 P 和 Fe 具有相似的生物学过程。层次聚类分析（HCA）的热图与主成分分析（PCA）的结果一致，反映了根和茎叶之间离子组的分化。此外，通过 PCA 鉴定了水稻植株对不同 As 物种处理的离子组差异。本研究表明，水稻植株的离子组特征足以检测对环境扰动的响应。离子组学与基因组学之间的关联研究对于进一步了解促进植物元素吸收或排斥的潜在机制是必要的。
