College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
College of Forestry, Henan Agriculture University, Zhengzhou, 450002, China.
Biometals. 2023 Oct;36(5):1157-1169. doi: 10.1007/s10534-023-00510-z. Epub 2023 May 17.
Ionomics and transcriptomics were applied to demonstrate response of rice to arsenite [As(III)] stress in the current study. Rice plants were cultured in nutrient solutions treated with 0, 100 and 500 μg/L As(III) coded as CK, As1 and As5, respectively. The rice ionomes exhibited discriminatory response to environmental disturbances. Solid evidence of the effects of As(III) stress on binding, transport or metabolism of P, K, Ca, Zn and Cu was obtained in this work. Differentially expressed genes (DEGs) in the shoots were identified in three datasets: As1 vs CK, As5 vs CK and As5 vs As1. DEGs identified simultaneously in two or three datasets were selected for subsequent interaction and enrichment analyses. Upregulation of genes involved in protein kinase activity, phosphorus metabolic process and phosphorylation were detected in the rice treated with As(III), resulting in the maintenance of P homeostasis in the shoots. Zn and Ca binding genes were up-regulated since excess As inhibited the translocation of Zn and Ca from roots to shoots. Increased expression of responsive genes including HMA, WRKY, NAC and PUB genes conferred As tolerance in the rice plants to cope with external As(III) stress. The results suggested that As(III) stress could disturb the uptake and translocation of macro and essential elements by rice. Plants could regulate the expression of corresponding genes to maintain mineral nutrient homeostasis for essential metabolic processes.
本研究应用离子组学和转录组学来研究水稻砷（As(III)）胁迫的响应。将水稻植株分别在含 0、100 和 500μg/L As(III)的营养液中培养，分别编码为 CK、As1 和 As5。水稻离子组对环境干扰表现出有区别的响应。本研究获得了确凿的证据，表明 As(III)胁迫对 P、K、Ca、Zn 和 Cu 的结合、转运或代谢有影响。在三个数据集（As1 vs CK、As5 vs CK 和 As5 vs As1）中鉴定了 shoots 中的差异表达基因（DEGs）。从两个或三个数据集同时鉴定的 DEGs 被选择用于后续的相互作用和富集分析。在 As(III)处理的水稻中，检测到参与蛋白激酶活性、磷代谢过程和磷酸化的基因上调，从而维持了 shoots 中的磷稳态。过量的 As 抑制了 Zn 和 Ca 从根部向地上部的转运，因此 Zn 和 Ca 结合基因被上调。响应基因（包括 HMA、WRKY、NAC 和 PUB 基因）的表达增加赋予了水稻对外部 As(III)胁迫的耐受性。结果表明，As(III)胁迫可能会干扰水稻对大量元素和必需元素的吸收和转运。植物可以通过调节相应基因的表达来维持矿物质营养的稳态，以满足必需的代谢过程。
