Department of Urology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
Department of Urology, The 458th Hospital of PLA, Guangzhou, China.
Neoplasma. 2020 Jan;67(1):78-84. doi: 10.4149/neo_2019_190224N158. Epub 2019 Nov 26.
Accumulating evidence indicates that eukaryotic translation elongation factor 1 alpha 1 (eEF1A1) is involved in cancer, while the clinical significance and the exact role of eEF1A1 in renal cell carcinoma (RCC) remain obscure. The aim of the present study was to evaluate the clinical significance of eEF1A1 in RCC and to investigate its effective mechanisms in order to identify a potential therapeutic target. The expression levels of eEF1A1 in RCC were explored by immunohistochemistry in tissues from 184 patients. eEF1A1 was knocked down, and cell proliferation and apoptosis were then investigated. The MAPK pathway-related proteins were detected by western blot. Our results revealed that eEF1A1 was highly expressed in RCC tissues and associated with poor prognosis. Knockdown of eEF1A1 attenuated proliferation and promoted the apoptosis of RCC cells. Furthermore, eEF1A1 knockdown decreased the phosphorylation level of AKT and ERK. In conclusion, eEF1A1 may serve as a valuable prognostic biomarker and promising therapeutic target of RCC.
越来越多的证据表明，真核翻译延伸因子 1α1（eEF1A1）参与癌症，而 eEF1A1 在肾细胞癌（RCC）中的临床意义和确切作用仍不清楚。本研究旨在评估 eEF1A1 在 RCC 中的临床意义，并探讨其有效的作用机制，以确定潜在的治疗靶点。通过免疫组织化学方法检测 184 例患者组织中 eEF1A1 的表达水平。敲低 eEF1A1 后，研究细胞增殖和凋亡情况。通过 Western blot 检测 MAPK 通路相关蛋白。结果表明，eEF1A1 在 RCC 组织中高表达，与预后不良相关。敲低 eEF1A1 可减弱 RCC 细胞的增殖并促进其凋亡。此外，eEF1A1 敲低降低了 AKT 和 ERK 的磷酸化水平。综上所述，eEF1A1 可能成为 RCC 有价值的预后生物标志物和有前途的治疗靶点。
