State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Horticulture, South China Agricultural University, Wushan Road 483, Tianhe District, Guangzhou 510642, China.
Key Laboratory of Loquat Germplasm Innovation and Utilization (Putian University), Fujian Province University, Putian 351100, China.
Int J Mol Sci. 2019 Nov 26;20(23):5933. doi: 10.3390/ijms20235933.
Flowering plants have evolved different flowering habits to sustain long-term reproduction. Most woody trees experience dormancy and then bloom in the warm spring, but loquat blooms in the cold autumn and winter. To explore its mechanism of flowering regulation, we cloned two homologous genes from 'Jiefanzhong' loquat ( Lindl.), namely, and . Sequence analysis revealed that the EjSVPs were typical MADS-box transcription factors and exhibited a close genetic relationship with other plant SVP/DORMANCY-ASSOCIATED MADS-BOX (DAM) proteins. The temporal and spatial expression patterns showed that and were mainly expressed in the shoot apical meristem (SAM) after the initiation of flowering; after reaching their highest level, they gradually decreased with the development of the flower until they could not be detected. expression levels were relatively high in young tissues, and expression levels were relatively high in young to mature transformed tissues. Interestingly, showed relatively high expression levels in various flower tissues. We analyzed the promoter regions and found that they did not contain the C-repeat/dehydration-responsive element. Finally, we overexpressed the in wild-type Col-0 and found no significant changes in the number of rosette leaves of ; however, overexpression of affected the formation of flower organs. In conclusion, were found to play an active role in the development of loquat flowering. These findings may provide a reference for exploring the regulation mechanisms of loquat flowering and the dormancy mechanisms of other plants.
开花植物进化出不同的开花习性以维持长期繁殖。大多数木本树木经历休眠，然后在温暖的春天开花，但枇杷在寒冷的秋冬开花。为了探索其开花调控机制，我们从‘解放钟’枇杷（ Lindl.）克隆了两个同源基因，即 和 。序列分析表明，EjSVPs 是典型的 MADS 框转录因子，与其他植物 SVP/DORMANCY-ASSOCIATED MADS-BOX (DAM) 蛋白具有密切的遗传关系。时空表达模式表明， 和 在开花启动后主要在茎尖分生组织（SAM）中表达；达到最高水平后，随着花的发育逐渐减少，直到无法检测到。 在年轻组织中的表达水平相对较高， 在年轻到成熟的转化组织中的表达水平相对较高。有趣的是， 在各种花组织中表现出相对较高的表达水平。我们分析了 启动子区域，发现它们不包含 C-repeat/dehydration-responsive element。最后，我们在野生型 Col-0 中过表达了 ，发现 中莲座叶的数量没有明显变化；然而， 的过表达影响了花器官的形成。总之， 被发现对枇杷开花发育起着积极的作用。这些发现可能为探索枇杷开花调控机制和其他植物休眠机制提供参考。
