Zhang Ji, Li Weibo, Yuan Qiaoyun, Zhou Jing, Zhang Jianmei, Cao Yufeng, Fu Guangbo, Hu Weicheng
Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University, Huaian, China.
Department of Urology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huaian, China.
Front Pharmacol. 2019 Nov 7;10:1331. doi: 10.3389/fphar.2019.01331. eCollection 2019.
20()-ginsenoside Rh2 (Rh2), a well-known protopanaxadiol-type ginsenoside from has especially gained attention for its anticancer activities on various types of human cancer cells. However, the molecular mechanism through which Rh2 promotes apoptosis in hepatocellular carcinoma (HePG2) cells is not known at the transcriptome level. Rh2 can specifically inhibit the proliferation of HePG2 in a dose- and time-dependent manner. Moreover, Rh2 can significantly increase the apoptosis which was related with an increase in protein expression levels of caspase-3, caspase-6, and poly (ADP-ribose) polymerase. Comparison of RNA-seq transcriptome profiles from control group and Rh2-treated group yielded a list of 2116 genes whose expression was significantly affected, which includes 971 up-regulated genes and 1145 down-regulated genes. The differentially expressed genes in p53 signaling pathway and DNA replication may have closely relationships to the cells apoptosis caused by Rh2 treatment. The results of qPCR validation showed that dynamic changes in mRNA, such as CDKN1A, CCND2, PMAIP1, GTSE1, and TP73.
20()-人参皂苷Rh2（Rh2）是一种来自[具体来源未提及]的著名原人参二醇型人参皂苷，因其对多种人类癌细胞的抗癌活性而备受关注。然而，在转录组水平上，Rh2促进肝癌（HePG2）细胞凋亡的分子机制尚不清楚。Rh2能以剂量和时间依赖性方式特异性抑制HePG2的增殖。此外，Rh2可显著增加细胞凋亡，这与半胱天冬酶-3、半胱天冬酶-6和聚（ADP-核糖）聚合酶的蛋白表达水平升高有关。对照组和Rh2处理组的RNA测序转录组图谱比较产生了一份2116个基因的列表，其表达受到显著影响，其中包括971个上调基因和1145个下调基因。p53信号通路和DNA复制中差异表达的基因可能与Rh2处理引起的细胞凋亡密切相关。qPCR验证结果显示了mRNA的动态变化，如CDKN1A、CCND2、PMAIP1、GTSE1和TP73。
