• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

子实体中一种水溶性杂多糖的制备、化学结构及免疫刺激活性

Preparation, chemical structure, and immunostimulatory activity of a water-soluble heteropolysaccharide from fruiting bodies.

作者信息

Gao Xiong, Zeng Ranhua, Ho Chi-Tang, Li Bin, Chen Shaodan, Xiao Chun, Hu Huiping, Cai Manjun, Chen Zhongzheng, Xie Yizhen, Wu Qingping

机构信息

State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.

College of Food Science, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou 510642, China.

出版信息

Food Chem X. 2022 Jan 15;13:100211. doi: 10.1016/j.fochx.2022.100211. eCollection 2022 Mar 30.

DOI:10.1016/j.fochx.2022.100211
PMID:35498979
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9039890/
Abstract

A water-soluble heteropolysaccharide (SGP2-1) was purified from fruiting bodies by anion-exchange chromatography and gel permeation chromatography. The structural characteristics were analyzed by high-performance gel permeation chromatography, high-performance liquid chromatography, Fourier transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy. The immunostimulatory activity was investigated using RAW 264.7 macrophages. Results showed that SGP2-1 with weight average molecular weight of 150.75 kDa was composed of mannose, glucose, and xylose. The backbone of SGP2-1 was mainly composed of → 4)--Glc-(1→, and the terminal group -d-Glc → was linked to the main chain by -6 position. SGP2-1 could significantly enhance pinocytic capacity, reactive oxygen species production, and cytokines secretion. SGP2-1 exerted immunomodulatory effects through interacting with toll-like receptor 2, and activating mitogen-activated protein kinase, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways. These findings indicated that SGP2-1 could be explored as a potential immunomodulatory agent for application in functional foods.

摘要

通过阴离子交换色谱和凝胶渗透色谱从子实体中纯化出一种水溶性杂多糖（SGP2-1）。采用高效凝胶渗透色谱、高效液相色谱、傅里叶变换红外光谱、气相色谱-质谱联用以及核磁共振光谱对其结构特征进行分析。利用RAW 264.7巨噬细胞研究其免疫刺激活性。结果表明，重均分子量为150.75 kDa的SGP2-1由甘露糖、葡萄糖和木糖组成。SGP2-1的主链主要由→4)--Glc-(1→组成，末端基团-d-Glc→通过-6位连接到主链上。SGP2-1可显著增强吞噬能力、活性氧生成以及细胞因子分泌。SGP2-1通过与Toll样受体2相互作用，并激活丝裂原活化蛋白激酶、磷脂酰肌醇-3-激酶/蛋白激酶B和核因子-κB信号通路发挥免疫调节作用。这些发现表明，SGP2-1可作为一种潜在的免疫调节剂应用于功能性食品中进行开发。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/9039890/710f1d8db612/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/9039890/b43d666da0a5/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/9039890/c6a85c77ed08/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/9039890/b35f91f43828/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/9039890/c037c2b87184/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/9039890/710f1d8db612/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/9039890/b43d666da0a5/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/9039890/c6a85c77ed08/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/9039890/b35f91f43828/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/9039890/c037c2b87184/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/9039890/710f1d8db612/gr5.jpg

相似文献

1
Preparation, chemical structure, and immunostimulatory activity of a water-soluble heteropolysaccharide from fruiting bodies.子实体中一种水溶性杂多糖的制备、化学结构及免疫刺激活性
Food Chem X. 2022 Jan 15;13:100211. doi: 10.1016/j.fochx.2022.100211. eCollection 2022 Mar 30.
2
Genistein promotes M1 macrophage apoptosis and reduces inflammatory response by disrupting miR-21/TIPE2 pathway.金雀异黄素通过破坏miR-21/TIPE2通路促进M1巨噬细胞凋亡并减轻炎症反应。
Saudi Pharm J. 2022 Jul;30(7):934-945. doi: 10.1016/j.jsps.2022.05.009. Epub 2022 May 23.
3
New inhibitors of nitric oxide production from the seeds of Myristica fragrans.肉豆蔻种子中一氧化氮生成的新型抑制剂。
Food Chem Toxicol. 2013 Dec;62:167-71. doi: 10.1016/j.fct.2013.08.046. Epub 2013 Aug 27.
4
Screening of synthetic PDE-5 inhibitors and their analogues as adulterants: analytical techniques and challenges.筛查合成 PDE-5 抑制剂及其类似物作为掺杂物：分析技术和挑战。
J Pharm Biomed Anal. 2014 Jan;87:176-90. doi: 10.1016/j.jpba.2013.04.037. Epub 2013 May 6.
5
Short-chain fatty acids reprogram metabolic profiles with the induction of reactive oxygen species production in human colorectal adenocarcinoma cells.短链脂肪酸通过诱导人结肠直肠腺癌细胞产生活性氧来重新编程代谢谱。
Comput Struct Biotechnol J. 2023 Feb 13;21:1606-1620. doi: 10.1016/j.csbj.2023.02.022. eCollection 2023.
6
Dingchuan tang essential oil inhibits the production of inflammatory mediators via suppressing the IRAK/NF-κB, IRAK/AP-1, and TBK1/IRF3 pathways in lipopolysaccharide-stimulated RAW264.7 cells.定喘汤精油通过抑制脂多糖刺激的RAW264.7细胞中的IRAK/NF-κB、IRAK/AP-1和TBK1/IRF3信号通路来抑制炎症介质的产生。
Drug Des Devel Ther. 2018 Sep 4;12:2731-2748. doi: 10.2147/DDDT.S160645. eCollection 2018.
7
Panax Notoginseng flower saponins (PNFS) inhibit LPS-stimulated NO overproduction and iNOS gene overexpression via the suppression of TLR4-mediated MAPK/NF-kappa B signaling pathways in RAW264.7 macrophages.三七花皂苷通过抑制RAW264.7巨噬细胞中TLR4介导的MAPK/NF-κB信号通路，抑制脂多糖刺激的一氧化氮过量产生和诱导型一氧化氮合酶基因的过表达。
Chin Med. 2015 Jul 1;10:15. doi: 10.1186/s13020-015-0045-x. eCollection 2015.
8
(E)-3-(3,4-Dimethoxyphenyl)-1-(5-hydroxy-2,2-dimethyl-2H-chromen-6-yl)prop-2-en-1-one ameliorates the collagen-arthritis via blocking ERK/JNK and NF-κB signaling pathway.（E）-3-（3,4-二甲氧基苯基）-1-（5-羟基-2,2-二甲基-2H-色烯-6-基）丙-2-烯-1-酮通过阻断ERK/JNK和NF-κB信号通路改善胶原性关节炎。
Int Immunopharmacol. 2013 Dec;17(4):1125-33. doi: 10.1016/j.intimp.2013.10.001. Epub 2013 Oct 14.
9
Structural characterization and immunomodulatory activity of a water-soluble polysaccharide from Ganoderma leucocontextum fruiting bodies.灵芝子实体水溶性多糖的结构表征及免疫调节活性。
Carbohydr Polym. 2020 Dec 1;249:116874. doi: 10.1016/j.carbpol.2020.116874. Epub 2020 Aug 10.
10
Immunoregulatory activity of a low-molecular-weight heteropolysaccharide from fruiting bodies and .来自子实体的一种低分子量杂多糖的免疫调节活性以及。 （原文表述似乎不完整）
Food Chem X. 2022 Apr 30;14:100321. doi: 10.1016/j.fochx.2022.100321. eCollection 2022 Jun 30.

引用本文的文献

1
Comparative Research of Antioxidant, Antimicrobial, Antiprotozoal and Cytotoxic Activities of Edible sp. Fruiting Body Extracts.食用蘑菇子实体提取物的抗氧化、抗菌、抗原虫和细胞毒性活性的比较研究。
Foods. 2025 Mar 25;14(7):1130. doi: 10.3390/foods14071130.
2
Purification, characterization, simulated gastrointestinal digestion and gut microbiota fermentation of a -directed mannoglucan from var. .来自变种的α-定向甘露葡聚糖的纯化、表征、模拟胃肠道消化及肠道微生物群发酵
Food Chem X. 2024 Jul 18;23:101671. doi: 10.1016/j.fochx.2024.101671. eCollection 2024 Oct 30.
3
Immunoregulatory activity of a low-molecular-weight heteropolysaccharide from fruiting bodies and .

本文引用的文献

1
Codonopsis pilosula polysaccharide in synergy with dacarbazine inhibits mouse melanoma by repolarizing M2-like tumor-associated macrophages into M1-like tumor-associated macrophages.党参多糖与达卡巴嗪协同作用通过将 M2 样肿瘤相关巨噬细胞重编程为 M1 样肿瘤相关巨噬细胞抑制小鼠黑色素瘤。
Biomed Pharmacother. 2021 Oct;142:112016. doi: 10.1016/j.biopha.2021.112016. Epub 2021 Aug 9.
2
Current trends in the anti-photoaging activities and mechanisms of dietary non-starch polysaccharides from natural resources.天然资源中膳食纤维的抗光老化活性及机制的研究进展。
Crit Rev Food Sci Nutr. 2022;62(32):9021-9035. doi: 10.1080/10408398.2021.1939263. Epub 2021 Jun 18.
3
来自子实体的一种低分子量杂多糖的免疫调节活性以及。 （原文表述似乎不完整）
Food Chem X. 2022 Apr 30;14:100321. doi: 10.1016/j.fochx.2022.100321. eCollection 2022 Jun 30.
Structural characterization and functional activity of an exopolysaccharide secreted by Rhodopseudomonas palustris GJ-22.
一株沼泽红假单胞菌 GJ-22 所分泌胞外多糖的结构特征与功能活性。
Int J Biol Macromol. 2021 Jan 15;167:160-168. doi: 10.1016/j.ijbiomac.2020.11.165. Epub 2020 Nov 27.
4
Surface morphology and protective effect of Hericium erinaceus polysaccharide on cyclophosphamide-induced immunosuppression in mice.糙皮侧耳多糖对环磷酰胺致免疫抑制小鼠的表面形态和保护作用。
Carbohydr Polym. 2021 Jan 1;251:116930. doi: 10.1016/j.carbpol.2020.116930. Epub 2020 Aug 25.
5
Structural characterization and immunomodulatory activity of a water-soluble polysaccharide from Ganoderma leucocontextum fruiting bodies.灵芝子实体水溶性多糖的结构表征及免疫调节活性。
Carbohydr Polym. 2020 Dec 1;249:116874. doi: 10.1016/j.carbpol.2020.116874. Epub 2020 Aug 10.
6
Immunomodulatory mechanism of a purified polysaccharide isolated from Isaria cicadae Miquel on RAW264.7 cells via activating TLR4-MAPK-NF-κB signaling pathway.蝉拟青霉中提取的一种纯化多糖通过激活 TLR4-MAPK-NF-κB 信号通路对 RAW264.7 细胞的免疫调节作用机制。
Int J Biol Macromol. 2020 Dec 1;164:4329-4338. doi: 10.1016/j.ijbiomac.2020.09.035. Epub 2020 Sep 11.
7
Immunomodulatory activities of polysaccharides from Ganoderma on immune effector cells.灵芝多糖对免疫效应细胞的免疫调节作用。
Food Chem. 2021 Mar 15;340:127933. doi: 10.1016/j.foodchem.2020.127933. Epub 2020 Aug 26.
8
Antioxidant and immunomodulatory activities in vitro of polysaccharides from bee collected pollen of Chinese wolfberry.枸杞蜂花粉多糖的体外抗氧化和免疫调节活性。
Int J Biol Macromol. 2020 Nov 15;163:190-199. doi: 10.1016/j.ijbiomac.2020.06.244. Epub 2020 Jun 30.
9
Characterization of a polysaccharide from Eupolyphaga sinensis walker and its effective antitumor activity via lymphocyte activation.中华真地鳖多糖的表征及其通过淋巴细胞激活的有效抗肿瘤活性
Int J Biol Macromol. 2020 Nov 1;162:31-42. doi: 10.1016/j.ijbiomac.2020.06.120. Epub 2020 Jun 15.
10
Purification and Characterization of a New CRISP-Related Protein from and Its Immunomodulatory Activity.从 中纯化和表征一种新型 CRISPR 相关蛋白及其免疫调节活性。
Mar Drugs. 2020 Jun 4;18(6):299. doi: 10.3390/md18060299.