State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, PR China; College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, PR China; College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Int J Biol Macromol. 2020 Apr 1;148:722-736. doi: 10.1016/j.ijbiomac.2020.01.178. Epub 2020 Jan 20.
The natural form of wild edible fungus is the fruiting body. The cultivation of fruiting bodies from sexual reproduction requires strict conditions and long periods. Some literatures have paid attentions on the mycelia prepared with liquid fermentation to alter fruiting bodies. Cordyceps militaris (C. militaris) is a kind of precious edible fungus. The polysaccharide is an important active ingredient in C. militaris. The manuscript aimed to evaluate the feasibility of alternative of mycelia to fruit bodies with studies of polysaccharides from C. militaris of different developmental stages. The two polysaccharides were separated. The chemical structure and inhibitory activity on α-glucosidase of polysaccharides were explored. The results indicated that the structure and inhibitory activity on α-glucosidase of polysaccharides with different developmental stages had significant differences. The polysaccharides from fruiting bodies had better inhibitory activity on α-glucosidase. It demonstrated that the mycelia of C. militaris from asexual reproduction with liquid fermentation can't be an effective substitute for fruiting bodies from sexual reproduction, from the perspective of polysaccharides.
野生食用菌的自然形态为子实体。有性生殖的子实体培养需要严格的条件和较长的时间。一些文献已经关注到利用液体发酵制备菌丝体来改变子实体。蛹虫草（Cordyceps militaris）是一种珍贵的食用菌。多糖是蛹虫草的重要活性成分。本研究旨在评估用菌丝体替代子实体的可行性，研究不同发育阶段蛹虫草的多糖。两种多糖被分离出来。探索了多糖的化学结构和对α-葡萄糖苷酶的抑制活性。结果表明，不同发育阶段的多糖在结构和对α-葡萄糖苷酶的抑制活性上存在显著差异。子实体多糖对α-葡萄糖苷酶的抑制活性更好。这表明，从多糖的角度来看，液体发酵的无性繁殖蛹虫草菌丝体不能作为有性生殖子实体的有效替代品。
