Kusche M, Lindahl U, Enerbäck L, Rodén L
Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala.
Biochem J. 1988 Aug 1;253(3):885-93. doi: 10.1042/bj2530885.
The oversulphated galactosaminoglycans synthesized by rat mucosal mast cells were isolated from the small intestine of animals infected with the nematode Nippostrongylus brasiliensis, which causes proliferation of these cells. The 35S-labelled polysaccharides were degraded by digestion with chondroitinase ABC, and the structures of the disaccharide products were determined by cleavage with mercuric acetate followed by electrophoretic characterization of the resultant sulphated monosaccharides. It was concluded that about half of the disulphated disaccharide units in the polysaccharide consisted of chondroitin sulphate E-type structures [GlcA-GalNAc(4,6-di-OSO3)], in which both sulphate groups were located on the N-acetylgalactosamine unit. The remainder consisted of isomeric structures with one sulphate group on the N-acetylgalactosamine residue and one on the hexuronic acid unit and presumably represented the dermatan sulphate-type sequence [IdoA(2-OSO3)-GalNAc(4-OSO3)].
从感染巴西日圆线虫的动物小肠中分离出大鼠黏膜肥大细胞合成的过度硫酸化的半乳糖胺聚糖，该线虫会导致这些细胞增殖。用软骨素酶ABC消化降解35S标记的多糖，通过乙酸汞裂解，随后对所得硫酸化单糖进行电泳表征来确定二糖产物的结构。得出的结论是，多糖中约一半的双硫酸化二糖单元由硫酸软骨素E型结构[GlcA-GalNAc(4,6-二-OSO3)]组成，其中两个硫酸基团都位于N-乙酰半乳糖胺单元上。其余部分由N-乙酰半乳糖胺残基上有一个硫酸基团且己糖醛酸单元上有一个硫酸基团的异构结构组成，推测代表硫酸皮肤素型序列[IdoA(2-OSO3)-GalNAc(4-OSO3)]。
