Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Environ Toxicol. 2020 Apr;35(4):457-467. doi: 10.1002/tox.22881. Epub 2019 Dec 2.
Cardamonin, a monomeric alkaloid, is isolated from Alpinia conchigera Griff and other natural plants. Recently, it has been focused on its anticancer activities, and no information showing its immune effects on leukemia mice was reported. In this study, we investigated the immune effects of cardamonin on WEHI-3 cell-generated leukemia mice. Forty BALB/c mice were randomly divided into four groups: Group I mice were normal animals and groups II-IV were leukemia. Group II mice, as a positive control, were administered with normal diet, and group III and IV mice were treated with 1 and 5 mg/kg of cardamonin, respectively, by intraperitoneal injection every 2 days for 14 days. The population of white blood cells, macrophage phagocytosis, and the proliferations of T and B cells were analyzed by flow cytometry. Another forty mice were also separated randomly into four groups for the determination of survival rate. Results showed that cardamonin did not affect body weight. Cardamonin decreased CD3, CD11b, and Mac-3 cell populations but increased CD19 number. Cardamonin enhanced phagocytic abilities of macrophages from the peripheral blood mononuclear cells of leukemia mice. Furthermore, cardamonin at 1 mg/kg treatment improved the survival rate of leukemia mice in vivo. Therefore, cardamonin could be applied for a leukemia therapeutic reagent at a defined dose.
小豆蔻明，一种单体生物碱，从益智 Alpinia conchigera Griff 和其他天然植物中分离得到。最近，它的抗癌活性受到了关注，但没有关于其对白血病小鼠免疫作用的信息报道。在本研究中，我们研究了小豆蔻明对 WEHI-3 细胞诱导的白血病小鼠的免疫作用。将 40 只 BALB/c 小鼠随机分为四组：第 I 组为正常动物，第 II-IV 组为白血病。第 II 组作为阳性对照组，给予正常饮食，第 III 和 IV 组分别通过腹腔注射给予 1 和 5mg/kg 的小豆蔻明，每 2 天一次，共 14 天。通过流式细胞术分析白细胞数量、巨噬细胞吞噬作用以及 T 和 B 细胞的增殖。另外 40 只小鼠也随机分为四组以确定存活率。结果表明，小豆蔻明不影响体重。小豆蔻明降低了 CD3、CD11b 和 Mac-3 细胞群体的数量，但增加了 CD19 的数量。小豆蔻明增强了白血病小鼠外周血单个核细胞中巨噬细胞的吞噬能力。此外，小豆蔻明在 1mg/kg 剂量下治疗可提高白血病小鼠的存活率。因此，小豆蔻明可作为一种特定剂量的白血病治疗试剂应用。
