Shang Hung-Sheng, Chen Chiung-Ju, Shih Yung-Luen, Peng Shu-Fen, Chen Yung-Liang, Liu Kuo-Ching, Huang Hsieh-Chou, Hsueh Shu-Ching, Chen Kuo-Wei, Lu Hsu-Feng, Lee Mei-Hui, Lee Ming-Zhe, Lu Kung-Wen
Graduate Institute of Clinical of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
Environ Toxicol. 2021 Jan;36(1):77-85. doi: 10.1002/tox.23013. Epub 2020 Sep 5.
Mangiferin is a naturally occurring polyphenol, widely distributed in Thymeraceae families, and presents pharmacological activity, including anti-cancer activities in many human cancer cell lines. Mangiferin has also been reported to affect immune responses; however, no available information concerning the effects of mangiferin on immune reactions in leukemia mice in vivo. In the present study, we investigated the effects of mangiferin on leukemia WEHI-3 cell generated leukemia BLAB/c mice. Overall, the experiments were divided into two parts, one part was immune responses experiment and the other was the survival rate experiment. The immune responses and survival rate study, 40 mice for each part, were randomly separated into five groups (N = 8): Group I was normal animals and groups II-V WEHI-3 cell generated leukemia mice. Group II mice were fed normal diet as a positive control; group III, IV, and V mice received mangiferin at 40, 80, and 120 mg/kg, respectively, by intraperitoneal injection every 2 days for 20 days. Leukocytes cell population, macrophage phagocytosis, and NK cell activities were analyzed by flow cytometry. Isolated splenocytes stimulated with lipopolysaccharide (LPS) and concanavalin A (Con A) were used to determine the proliferation of B and T cells, respectively, and subsequently were analyzed by flow cytometry. Results indicated that mangiferin significantly increased body weight, decreased the liver and spleen weights of leukemia mice. Mangiferin also increased CD3 T-cell and CD19 B cell population but decreased Mac-3 macrophage and CD11b monocyte. Furthermore, mangiferin decreased phagocytosis of macrophages from PBMC and peritoneal cavity at 40, 80, and 120 mg/kg treatment. However, it also increased NK cell activity at 40 and 120 mg/kg treatment. There were no effects on T and B cell proliferation at three examined doses. In survival rate studies, mangiferin significantly elevated survival rate at 40 and 120 mg/kg treatment of leukemia mice in vivo.
芒果苷是一种天然存在的多酚，广泛分布于藤黄科植物中，并具有药理活性，包括在许多人类癌细胞系中的抗癌活性。也有报道称芒果苷会影响免疫反应；然而，关于芒果苷对白血病小鼠体内免疫反应影响的现有信息尚少。在本研究中，我们调查了芒果苷对白血病WEHI-3细胞诱导的白血病BLAB/c小鼠的影响。总体而言，实验分为两部分，一部分是免疫反应实验，另一部分是存活率实验。免疫反应和存活率研究中，每部分40只小鼠被随机分为五组（N = 8）：第一组为正常动物，第二至五组为WEHI-3细胞诱导的白血病小鼠。第二组小鼠喂食正常饮食作为阳性对照；第三、四、五组小鼠分别以40、80和120 mg/kg的剂量，每2天腹腔注射一次芒果苷，共注射20天。通过流式细胞术分析白细胞细胞群、巨噬细胞吞噬作用和NK细胞活性。分别用脂多糖（LPS）和伴刀豆球蛋白A（Con A）刺激分离的脾细胞，以测定B细胞和T细胞的增殖，随后通过流式细胞术进行分析。结果表明，芒果苷显著增加了白血病小鼠的体重，降低了其肝脏和脾脏重量。芒果苷还增加了CD3 T细胞和CD19 B细胞数量，但减少了Mac-3巨噬细胞和CD11b单核细胞数量。此外，在40、80和120 mg/kg剂量处理下，芒果苷降低了外周血单核细胞（PBMC）和腹腔巨噬细胞的吞噬作用。然而，在40和120 mg/kg剂量处理下，它也增加了NK细胞活性。在所检测的三个剂量下，对T细胞和B细胞增殖均无影响。在存活率研究中，在体内以40和120 mg/kg剂量处理白血病小鼠时，芒果苷显著提高了存活率。
