Faculty of Chemical and Process Engineering Technology, College of Engineering Technology, Universiti Malaysia Pahang, 26300 Gambang, Kuantan, Pahang, Malaysia.
Faculty of Chemical and Process Engineering Technology, College of Engineering Technology, Universiti Malaysia Pahang, 26300 Gambang, Kuantan, Pahang, Malaysia.
Ultrason Sonochem. 2020 Apr;62:104876. doi: 10.1016/j.ultsonch.2019.104876. Epub 2019 Nov 15.
Phenolic acids of oak gall were extracted using ultrasonic-probe assisted extraction (UPAE) method in the presence of ionic liquid. It was compared with classical ultrasonic-bath assisted extraction (CUBAE) and conventional aqueous extraction (CAE) method, with and without the presence of ionic liquid. Remarkably, the UPAE method yielded two-fold higher extraction yield with the presence of ionic liquid, resulting 481.04 mg/g for gallic acids (GA) and 2287.90 mg/g for tannic acids (TA), while a decreased value of 130.36 mg/g for GA and 1556.26 mg/g for TA were resulted with the absence of ionic liquid. Intensification process resulted the highest yield of 497.34 mg/g and 2430.48 mg/g for GA and TA, respectively, extracted at temperature 50 °C with sonication intensity of 8.66 W/cm and 10% duty cycle, diluted in ionic liquid, 1-Butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide, [Bmim][TfN] at concentration of 0.10 M with sample-to-solvent ratio 1:10 for 8 h. Peleg's model successfully predicted the UPAE process confirming that extraction capacity is the controlling factor in extracting phenolic acids. Hence, it can be concluded that UPAE method and ionic liquid have synergistic effect as it effectively enhanced the extraction efficiency to increase the bioactive constituents yield.
采用超声探针辅助提取（UPAE）法，在离子液体存在的情况下提取橡实中的酚酸。将其与经典超声浴辅助提取（CUBAE）和常规水提取（CAE）法进行了比较，同时也比较了有无离子液体存在的情况。值得注意的是，在离子液体存在的情况下，UPAE 法的提取产率提高了两倍，得到了 481.04mg/g 的没食子酸（GA）和 2287.90mg/g 的单宁酸（TA），而在没有离子液体存在的情况下，GA 的提取产率降低到 130.36mg/g，TA 的提取产率降低到 1556.26mg/g。强化过程使 GA 和 TA 的提取产率分别达到最高，分别为 497.34mg/g 和 2430.48mg/g，在 50°C 下提取，超声强度为 8.66W/cm，占空比为 10%，稀释在离子液体 1-丁基-3-甲基咪唑双（三氟甲烷磺酰基）亚胺中，[Bmim][TfN]浓度为 0.10M，样品与溶剂比为 1:10，提取 8h。Peleg 模型成功预测了 UPAE 过程，证实了提取能力是提取酚酸的控制因素。因此，可以得出结论，UPAE 法和离子液体具有协同作用，因为它可以有效地提高提取效率，增加生物活性成分的产率。
