Zhou Qiao, Wu Xun, Liu Yingmin, Wang Xin, Ling Xiufeng, Ge Hongshan, Zhang Junqiang
Department of Reproduction, the Affiliated Obstetrics and Gynecology Hospital, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, China.
State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
Andrologia. 2020 Mar;52(2):e13491. doi: 10.1111/and.13491. Epub 2019 Dec 3.
We conducted this study for the purpose of evaluating the protective mechanisms of curcumin against oxidative stress in asthenozoospermic individuals. Asthenozoospermic individuals were grouped into AS group, curcumin treatment group and brusatol + curcumin treatment group. The sperm motility was measured by computer-aided sperm analysis. We conducted flow cytometry and spectrophotometry to assess the levels of reactive oxygen species (ROS) and malondialdehyde (MDA). Chlortetracycline (CTC) was used to examine the acrosomal reaction of spermatozoa. Also, Western blotting was carried to measure antioxidant gene Nrf2 (nuclear factor erythroid 2-related factor) expression level. As our results shown, treatment with curcumin significantly decreased ROS formation and MDA production, compared with spermatozoa of AS group; however, Nrf2 inhibitor, Brusatol, inhibited Nrf2 expression and sperm function. Our results have shown that curcumin might protect spermatozoa by regulating Nrf2 level.
我们开展这项研究的目的是评估姜黄素对弱精子症患者氧化应激的保护机制。弱精子症患者被分为AS组、姜黄素治疗组和布沙替尼+姜黄素治疗组。通过计算机辅助精子分析测量精子活力。我们进行了流式细胞术和分光光度法来评估活性氧（ROS）和丙二醛（MDA）的水平。使用金霉素（CTC）检测精子的顶体反应。此外，通过蛋白质免疫印迹法检测抗氧化基因Nrf2（核因子红细胞2相关因子）的表达水平。结果显示，与AS组精子相比，姜黄素治疗显著降低了ROS的形成和MDA的产生；然而，Nrf2抑制剂布沙替尼抑制了Nrf2的表达和精子功能。我们的结果表明，姜黄素可能通过调节Nrf2水平来保护精子。
