Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, NY, USA.
Environmental Studies Department, New York University, New York, NY, USA.
Inhal Toxicol. 2019 Sep-Oct;31(11-12):399-408. doi: 10.1080/08958378.2019.1698678. Epub 2019 Dec 4.
To (1) design and build a low-cost exposure chamber system for whole-body exposure of rodents to electronic cigarette aerosol, (2) provide detailed instructions with particular focus on automated e-cigarette activation, and (3) develop a simple mathematical model for aerosol levels in the exposure chamber. We built the system with standard laboratory equipment and an open-source electronics platform (Arduino) for e-cigarette activation. Arduino is used to control a solenoid, which pushes the activation button of so-called "Mod" e-cigarettes, and a pump to move the aerosol from the mouthpiece of the e-cigarette into the chamber. For "Pods" and "Cigalikes," the solenoid is not used as they are activated by the vacuum created by the pump. Aerosol concentrations were measured with a light-scattering laser photometer. The system allows varying the air exchange rate, monitoring aerosol levels, and programing arbitrary puff topography. Aerosol concentrations observed for different chamber operating conditions (puff time and period, e-cigarette power output, air exchange rate) were consistent with the mathematical model. Our low-cost exposure chamber can be used in animal experimental studies of the health effects of e-cigarettes. Our model allows estimating design parameters such as chamber volume, air exchange rate, and puff period.
（1）设计并建立一个用于啮齿动物全身暴露于电子烟气溶胶的低成本暴露舱系统，（2）提供详细的说明，特别关注电子烟的自动激活，以及（3）开发暴露舱内气溶胶水平的简单数学模型。我们使用标准实验室设备和用于电子烟激活的开源电子平台（Arduino）构建了该系统。Arduino 用于控制电磁阀，电磁阀推动所谓的“Mod”电子烟的激活按钮，以及一个泵将气溶胶从电子烟的吸嘴吸入舱内。对于“Pods”和“Cigalikes”，不使用电磁阀，因为它们是由泵产生的真空激活的。使用光散射激光光度计测量气溶胶浓度。该系统允许改变空气交换率，监测气溶胶水平，并编程任意的抽吸模式。对于不同的腔室操作条件（抽吸时间和周期、电子烟功率输出、空气交换率）观察到的气溶胶浓度与数学模型一致。我们的低成本暴露舱可用于电子烟健康影响的动物实验研究。我们的模型允许估算设计参数，如腔室体积、空气交换率和抽吸周期。
