The First Affiliated Hospital, Biomedical Translational Research Institute, Jinan University, Guangzhou, Guangdong 510632, China.
Food Funct. 2020 Jan 29;11(1):347-357. doi: 10.1039/c9fo02000d.
Hypotaurine, an important sulfur-containing and nonpeptidic amino acid, is a precursor of taurine and an antioxidant. Our previous study indicated that hypotaurine levels are associated with the ageing of Caenorhabditis elegans (C. elegans). However, whether hypotaurine plays a role in the lifespan regulation of C. elegans and the mechanism remains undetermined. Here, we found that hypotaurine enhances oxidative stress resistance and ameliorates ageing in C. elegans. Our results show that hypotaurine regulates a variety of pathways and leads to the upregulation of some age-related genes to extend lifespan. We also found that the stress response-related transcription factors DAF-16/FOXO and SKN-1/NRF2 contribute to the beneficial longevity conferred by hypotaurine. Moreover, our results demonstrate that hypotaurine induced lifespan extension by regulating the insulin/IGF-1 signaling (IIS) pathway, the reproductive signaling pathway and DR-like mechanisms. Additionally, our results also indicated that mitochondrial function also plays a crucial role in the lifespan extension induced by hypotaurine. Taken together, these data indicate that hypotaurine delays the ageing of C. elegans, due, at least in part, to its antioxidant activity, which in turn regulates IIS, and reproductive and DR-related pathways, thereby inducing the activity of the transcription factors DAF-16 and SKN-1.
次牛磺酸是一种重要的含硫非肽类氨基酸，是牛磺酸和抗氧化剂的前体。我们之前的研究表明，次牛磺酸水平与秀丽隐杆线虫（C. elegans）的衰老有关。然而，次牛磺酸是否在 C. elegans 的寿命调节中发挥作用以及其机制仍未确定。在这里，我们发现次牛磺酸增强了秀丽隐杆线虫的氧化应激抗性并改善了其衰老。我们的结果表明，次牛磺酸调节多种途径，并上调一些与年龄相关的基因以延长寿命。我们还发现，应激反应相关转录因子 DAF-16/FOXO 和 SKN-1/NRF2 有助于次牛磺酸赋予的有益长寿。此外，我们的结果表明，次牛磺酸通过调节胰岛素/IGF-1 信号（IIS）途径、生殖信号途径和 DR 样机制来延长寿命。此外，我们的结果还表明，线粒体功能在次牛磺酸诱导的寿命延长中也起着至关重要的作用。综上所述，这些数据表明次牛磺酸延缓了秀丽隐杆线虫的衰老，至少部分原因是其抗氧化活性，这反过来又调节了 IIS 以及生殖和 DR 相关途径，从而诱导转录因子 DAF-16 和 SKN-1 的活性。
