Rosamond W D, Savaiano D A
Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108.
Dig Dis Sci. 1988 Nov;33(11):1397-402. doi: 10.1007/BF01536994.
The long-term maintenance and acute induction of sucrase activity were studied under a variety of defined dietary conditions during the adult life-span of the Sprague-Dawley rat. Rats were fed chow, 74% carbohydrate, 56% carbohydrate, or 22% carbohydrate diets, from 44 to 106 weeks of age. Sucrase specific activity was measured in enterocytes isolated from the proximal, middle, and distal small intestine at 52, 86, and 104 weeks of age. Sucrase specific activity was correlated with the carbohydrate content of the diet but not affected by age throughout the adult life-span. In a second experiment, the acute induction of sucrase following a 48-hr fast was studied in 58- and 116-week-old rats. The increase in sucrase specific activity following refeeding was not different in the adult and aged rats. The results suggest that the carbohydrate-digesting capacity of the small intestine enterocyte of the Sprague-Dawley rat is not impaired during the aging process.
在斯普拉格-道利大鼠成年期的各种特定饮食条件下，研究了蔗糖酶活性的长期维持和急性诱导情况。大鼠在44至106周龄期间分别喂食普通饲料、碳水化合物含量为74%、56%或22%的饲料。在52、86和104周龄时，测定从近端、中端和远端小肠分离的肠细胞中的蔗糖酶比活性。蔗糖酶比活性与饮食中的碳水化合物含量相关，但在整个成年期不受年龄影响。在第二个实验中，研究了58周龄和116周龄大鼠禁食48小时后蔗糖酶的急性诱导情况。再喂食后蔗糖酶比活性的增加在成年大鼠和老年大鼠中没有差异。结果表明，斯普拉格-道利大鼠小肠肠细胞的碳水化合物消化能力在衰老过程中没有受损。
