Xue Fang, Xu Yichi, Song Yizuo, Zhang Wenwen, Li Ruyi, Zhu Xueqiong
Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, People's Republic of China.
Drug Des Devel Ther. 2019 Nov 18;13:3919-3928. doi: 10.2147/DDDT.S219788. eCollection 2019.
To investigate the effect of sevoflurane on the progression of cervical cancer cells, and to explore its effect on the cisplatinum (DDP) sensitivity in cervical cancer cells and underlying mechanism.
Siha and Hela cervical cancer cells were cultured and treated with 3% sevoflurane, 10 μmol/L DDP, or the co-treatment of sevoflurane and DDP, respectively. Cell proliferation was evaluated by the CCK8 assay. Cell apoptosis was assessed by flow cytometry. Cell migration was detected by wound healing assay. The expression of B-cell lymphoma-2 (BCL-2), B-cell lymphoma-2 associated X (BAX), Ezrin, matrix metalloproteinase 2 (MMP2), lung resistance-related protein (LRP), multiple drug resistance protein 1 (MRP1), glutathione-S-transferase-π (GST-π), and P glycoprotein (P-gp) protein was determined by Western blotting.
After treated with sevoflurane, cell proliferation and migration rate in Siha and Hela cells were significantly elevated, while cell apoptosis was decreased. In addition, the expression of migration-related protein Ezrin and MMP2 was increased accordingly, apoptotic-related protein BCL-2 expression was also increased while BAX protein expression was decreased after sevoflurane treatment. The proliferation, migration rate, and apoptosis of Siha and Hela cells in sevoflurane plus DDP group were not significantly different with those in DDP group. There was no significant difference in apoptotic-related protein, migration-related protein, and drug resistance-associated proteins expression between DDP treatment group and combined treatment group.
Sevoflurane promotes the progression but has no effect on the cisplatinum sensitivity in cervical cancer cells.
探讨七氟醚对宫颈癌细胞进展的影响，并探究其对宫颈癌细胞顺铂（DDP）敏感性的影响及潜在机制。
培养Siha和Hela宫颈癌细胞，分别用3%七氟醚、10 μmol/L DDP或七氟醚与DDP联合处理。通过CCK8法评估细胞增殖。通过流式细胞术评估细胞凋亡。通过伤口愈合试验检测细胞迁移。通过蛋白质免疫印迹法测定B细胞淋巴瘤-2（BCL-2）、B细胞淋巴瘤-2相关X蛋白（BAX）、埃兹蛋白、基质金属蛋白酶2（MMP2）、肺耐药相关蛋白（LRP）、多药耐药蛋白1（MRP1）、谷胱甘肽-S-转移酶-π（GST-π）和P糖蛋白（P-gp）的表达。
七氟醚处理后，Siha和Hela细胞的增殖和迁移率显著升高，而细胞凋亡减少。此外，七氟醚处理后，迁移相关蛋白埃兹蛋白和MMP2的表达相应增加，凋亡相关蛋白BCL-2表达也增加，而BAX蛋白表达减少。七氟醚加DDP组Siha和Hela细胞的增殖、迁移率和凋亡与DDP组无显著差异。DDP处理组和联合处理组在凋亡相关蛋白、迁移相关蛋白和耐药相关蛋白表达方面无显著差异。
七氟醚促进宫颈癌细胞进展，但对宫颈癌细胞的顺铂敏感性无影响。
