Department of Pharmacy, The first affiliated hospital of Anhui university of Chinese medicine, 117 Meishan Road, Hefei, China.
Department of Nephrology, The first affiliated hospital of Anhui university of Chinese medicine, 117 Meishan Road, Hefei, China.
Gene. 2020 Feb 20;728:144279. doi: 10.1016/j.gene.2019.144279. Epub 2019 Dec 9.
Chronic glomerulonephritis (CGN) is the most common form of primary glomerular disease. Qi Teng Xiao Zhuo granules have been proposed as a prescription of traditional Chinese medicine (TCM) for treatment of CGN, however,the comprehensive molecular mechanism underlying this therapeutic effectremains unclear to date. Our study aimed to evaluate and analyze the possible roles and molecular mechanisms of Qi Teng Xiao Zhuo granule-mediated treatment of CGN induced by adriamycin in rats.
RNA-sequencing and real-time polymerase chain reaction (RT-PCR) were applied to identify specifically expressed long noncoding RNAs (lncRNAs) in glomerular tissues of rats from the control group, adriamycin-induced group, and Qi Teng Xiao Zhuo granules group (n = 3). Differentially expressed lncRNAs and mRNAs (messengerRNAs) were screened out among the 3 groups. Gene ontology (GO) and pathway enrichment analyses were performed to analyze the biological functions and pathways for mRNAs. LncRNA-mRNA co-expression network was constructed to analyse for the genes. The protein-protein interaction (PPI) network was visualized.
A total of 473 significantly up and down-regulated lncRNAs, 753 up and down-regulated mRNAs were identified. Additionally, it is worth noting that TOP2a (topoisomerase (DNA) II alpha), with the highest connectivity degree in PPI network, was enriched in variouskinds of pathways. Coding-non-coding gene co-expression networks (CNC network) were drawn based on the correlation analysis between lncRNAs and mRNAs. Ten lncRNAs, NONRATT009275.2, NONRATT025409.2, NONRATT025419.2, MSTRG.7681.1, ENSRNOT00000084373, NONRATT000512.2, NONRATT006734.2, ENSRNOT00000084386, NONRATT021738.2, ENSRNOT00000084080, were selected to analyse the relationship between LncRNAs and Qi Teng Xiao Zhuo granules via the CNC network (Coding-non-coding gene co-expression networks) and GO analysis. Real-time PCR results confirmed that the six lncRNAs were specifically expressed in the Qi Teng Xiao Zhuo granules rats.
The ten lncRNAs might play important roles in the Qi Teng Xiao Zhuo granules treatment of CGN. Key genes, such as Ptprc (protein tyrosine phosphatase, receptor type, C), TOP2a, Fos (FBJ osteosarcoma oncogene), Myc (myelocytomatosis oncogene), etc, may be crucial biomarkers for Qi Teng Xiao Zhuo granules.
慢性肾小球肾炎（CGN）是原发性肾小球疾病中最常见的形式。芪藤消浊颗粒已被提议作为一种中药（TCM）处方用于治疗 CGN，然而，其治疗效果的综合分子机制至今仍不清楚。本研究旨在评估和分析芪藤消浊颗粒对阿霉素诱导的大鼠 CGN 的可能作用和分子机制。
采用 RNA 测序和实时聚合酶链反应（RT-PCR）技术，鉴定对照组、阿霉素诱导组和芪藤消浊颗粒组大鼠肾小球组织中特异性表达的长非编码 RNA（lncRNA）（n=3）。筛选出 3 组间差异表达的 lncRNA 和信使 RNA（mRNA）。对 mRNA 进行基因本体论（GO）和途径富集分析，以分析其生物学功能和途径。构建 lncRNA-mRNA 共表达网络以分析基因。可视化蛋白质-蛋白质相互作用（PPI）网络。
共鉴定出 473 个显著上调和下调的 lncRNA、753 个上调和下调的 mRNA。此外，值得注意的是，在 PPI 网络中，拓扑异构酶（DNA）IIα（TOP2a）的连接度最高，它富集在各种途径中。基于 lncRNA 和 mRNA 之间的相关性分析，绘制编码-非编码基因共表达网络（CNC 网络）。根据 CNC 网络和 GO 分析，选择 10 个 lncRNA（NONRATT009275.2、NONRATT025409.2、NONRATT025419.2、MSTRG.7681.1、ENSRNOT00000084373、NONRATT000512.2、NONRATT006734.2、ENSRNOT00000084386、NONRATT021738.2、ENSRNOT00000084080）分析 LncRNA 与芪藤消浊颗粒的关系。实时 PCR 结果证实，这 6 个 lncRNA 在芪藤消浊颗粒大鼠中特异性表达。
这 10 个 lncRNA 可能在芪藤消浊颗粒治疗 CGN 中发挥重要作用。Ptprc（蛋白酪氨酸磷酸酶，受体型，C）、TOP2a、Fos（FBJ 骨肉瘤癌基因）、Myc（髓母细胞瘤癌基因）等关键基因可能是芪藤消浊颗粒的关键生物标志物。
