Martschini Andrea, Kostelac Anja, Haltrich Dietmar, Peterbauer Clemens K
Food Biotechnology Laboratory, Department of Food Science and Technology, BOKU University, 1190 Vienna, Austria.
Doctoral Programme Molecular Biotechnology of Proteins BioToP, BOKU University, 1190 Vienna, Austria.
Biomolecules. 2024 Nov 26;14(12):1510. doi: 10.3390/biom14121510.
Pyranose oxidase (POx) is an FAD-dependent oxidoreductase and belongs to the glucose-methanol-choline (GMC) superfamily of oxidoreductases. As recently reported, POxs and FAD-dependent -glycoside oxidases (CGOxs) share the same sequence space, and phylogenetic analysis of actinobacterial sequences belonging to this shared sequence space showed that it can be divided into four clades. Here, we report the biochemical characterization of a POx/CGOx from sp. 3H14 (POx), belonging to the hitherto unexplored clade II of actinobacterial POx/CGOx. Overall, POx demonstrates comparable features to POxs/CGOxs of clades III and IV, including the preference for glycosides over monosaccharides as electron donors. However, as POx efficiently oxidizes the -glycoside aspalathin as well as the -glycoside phlorizin, it shows activity with yet another set of glycoside structures compared to other POx/CGOx members.
吡喃糖氧化酶（POx）是一种依赖黄素腺嘌呤二核苷酸（FAD）的氧化还原酶，属于氧化还原酶的葡萄糖-甲醇-胆碱（GMC）超家族。正如最近报道的那样，POx和依赖FAD的β-糖苷氧化酶（CGOx）共享相同的序列空间，对属于这个共享序列空间的放线菌序列进行系统发育分析表明，它可以分为四个进化枝。在这里，我们报道了来自sp. 3H14的一种POx/CGOx（POx）的生化特性，该酶属于放线菌POx/CGOx迄今未被探索的进化枝II。总体而言，POx表现出与进化枝III和IV的POx/CGOx相当的特性，包括相对于单糖更倾向于以糖苷作为电子供体。然而，由于POx能有效氧化β-糖苷阿斯巴甜以及β-糖苷根皮苷，与其他POx/CGOx成员相比，它对另一组糖苷结构表现出活性。
