Department of Anesthesiology, Shanxi Provincial People's Hospital, Taiyuan 030012, China.
Department of Anesthesiology, Dongying Second People's Hospital, Dongying 257335, China.
J Photochem Photobiol B. 2020 Jan;202:111716. doi: 10.1016/j.jphotobiol.2019.111716. Epub 2019 Nov 18.
Though anesthetic drug delivery system and drug vehicles is generally applied for pain relief, there are have many difficulties and issues due to its short duration carrier and low biocompatibility, effectiveness at the conditions of inflammation at acidic pH. To resolve this issue, we have designed and developed the dual (pH and temperature) responsive bio-nanomaterial to improve the efficiency anesthetic drug delivery system. Chitosan is a unique class of biomaterials that is widely used in medical devices. The surface engineering of ZnFeO nanoparticles was performed by coating with chitosan using simple precipitation method. Then, multi-active anesthetic drug (Lidocaine) was loaded into nano-ferrite to form a drug delivery vehicle. The prepared drug-vesicle was characterized by using XRD, FTIR, SEM, XPS and TGA analysis. XRD analysis proved the face center cubic structure of zinc nanoferrite. The sustained delivery of Lidocaine (LDC) from CS coated nanoferrite (CS/ZnFeO) was stimulated by pH and temperature responsive characteristics of vesicles. The in vitro cytotoxicity of the CS/ZnFeO particles towards fibroblast cells was analyzed by using MTT assay. The drug loaded CS/ZnFeO particles exhibit high biocompatibility and sustained drug release in the physiological pH environment (4.8, 5.5 and 7.4) and temperature responsive (25 and 37 °C) of normal tissues and also drug loading efficiency was measured.
虽然麻醉药物输送系统和药物载体通常用于缓解疼痛，但由于其载体的半衰期短和生物相容性低，在酸性 pH 值条件下的炎症情况下的效果有限，因此存在许多困难和问题。为了解决这个问题，我们设计并开发了双（pH 和温度）响应生物纳米材料，以提高麻醉药物输送系统的效率。壳聚糖是一类独特的生物材料，广泛应用于医疗器械中。通过简单的沉淀法用壳聚糖对 ZnFeO 纳米粒子进行表面工程处理。然后，将多活性麻醉药物（利多卡因）负载到纳米铁氧体中，形成药物输送载体。通过 XRD、FTIR、SEM、XPS 和 TGA 分析对制备的药物囊泡进行了表征。XRD 分析证明了锌纳米铁氧体的面心立方结构。利多卡因（LDC）从 CS 包覆的纳米铁氧体（CS/ZnFeO）中的持续释放受到囊泡的 pH 和温度响应特性的刺激。通过 MTT 分析研究了 CS/ZnFeO 颗粒对成纤维细胞的体外细胞毒性。载药 CS/ZnFeO 颗粒在生理 pH 值环境（4.8、5.5 和 7.4）和正常组织的温度响应（25 和 37°C）下表现出高生物相容性和持续的药物释放，并且还测量了药物负载效率。
