Department of Orthopaedic Surgery, Center for Osteonecrosis and Joint Preserving & Reconstruction, China-Japan Friendship Hospital, Beijing 100029, China.
Department of Orthopaedic Surgery, Peking University China-Japan Friendship School of Clinical Medicine, Beijing 100029, China.
Biomed Res Int. 2019 Nov 20;2019:1302015. doi: 10.1155/2019/1302015. eCollection 2019.
The immunologic factors have been implicated in the pathogenesis of osteonecrosis. We aimed to investigate the potential role of immune regulatory cells in the development of osteonecrosis of femoral head (ONFH). Sixty-seven patients diagnosed with ONFH and fifty-eight age-, height-, and weight-matched healthy subjects were included in this retrospective study between September 2015 and September 2018. The flow cytometry was used to test the count, percentage, and ratio of T and B lymphocyte subsets in peripheral blood. The T and B lymphocyte levels were compared among different ARCO stages, CJFH types, and etiology groups. The total lymphocyte count, CD3T cells, Ts cells (CD3CD8), B-1 cell count, and B-1 cells (CD5CD19) were significantly higher in the patients with ONFH than those in the control subjects. The percentage of T lymphocytes in the patients with ARCO IV stage was significantly smaller than that in the ONFH patients with ARCO II and III stages. The percentage of inhibitory T lymphocytes in patients with CJFH type L3 was significantly smaller than that in the patients with types L1 and L2. In terms of the different ONFH etiologies, the total lymphocyte count and Ts cells (CD3CD8) were significantly lower in the ONFH patients induced by excessive alcohol intake than those in the idiopathic ONFH patients. Our results seem to indicate that immune regulatory cells, such as T and B lymphocytes, play an important role in the pathogenesis of ONFH. The development and progression of ONFH may be associated with immune system imbalance.
免疫因素与骨坏死的发病机制有关。我们旨在研究免疫调节细胞在股骨头坏死（ONFH）发展中的潜在作用。本回顾性研究纳入了 2015 年 9 月至 2018 年 9 月期间的 67 例确诊为 ONFH 的患者和 58 名年龄、身高和体重匹配的健康对照者。采用流式细胞术检测外周血 T、B 淋巴细胞亚群的计数、百分比和比值。比较了不同 ARCO 分期、CJFH 类型和病因组之间的 T、B 淋巴细胞水平。ONFH 患者的总淋巴细胞计数、CD3T 细胞、Ts 细胞（CD3CD8）、B-1 细胞计数和 B-1 细胞（CD5CD19）均明显高于对照组。ARCO IV 期患者的 T 淋巴细胞百分比明显小于 ARCO II 和 III 期患者。L3 型 CJFH 患者的抑制性 T 淋巴细胞百分比明显小于 L1 和 L2 型患者。就不同的 ONFH 病因而言，过度饮酒引起的 ONFH 患者的总淋巴细胞计数和 Ts 细胞（CD3CD8）明显低于特发性 ONFH 患者。我们的结果似乎表明，T 和 B 淋巴细胞等免疫调节细胞在 ONFH 的发病机制中起重要作用。ONFH 的发展和进展可能与免疫系统失衡有关。
