miR-222-3p 的过表达通过抑制 PPP2R2A 促进弥漫性大 B 细胞淋巴瘤细胞的增殖并抑制其凋亡。
Overexpression of miR-222-3p Promotes the Proliferation and Inhibits the Apoptosis of Diffuse Large B-Cell Lymphoma Cells via Suppressing PPP2R2A.
机构信息
Department of Hematology, Shouguang People's Hospital, Shouguang, Shandong, China.
CT Magnetic Resonance Imaging Room, Shouguang People's Hospital, Shouguang, Shandong, China.
出版信息
Technol Cancer Res Treat. 2019 Jan-Dec;18:1533033819892256. doi: 10.1177/1533033819892256.
PURPOSE
This study aimed to investigate the effects of microRNA-222-3p on activated B cell-like-type diffuse large B-cell lymphoma cells and the regulatory relationship between microRNA-222-3p and phosphatase 2 regulatory subunit B alpha.
METHOD
The expression of microRNA-222-3p was detected in activated B cell-like-type diffuse large B-cell lymphoma tissues and cells by quantitative reverse transcription polymerase chain reaction. The regulatory effects of microRNA-222-3p on the proliferation, invasion, and apoptosis of activated B cell-like-type diffuse large B-cell lymphoma cells were analyzed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), colony formation, flow cytometry, and Transwell assay. The regulatory relationship between microRNA-222-3p and phosphatase 2 regulatory subunit B alpha was determined by luciferase reporter gene and RNA pull-down assay. In addition, the effects of microRNA-222-3p on tumor growth were further analyzed in mice.
RESULTS
MicroRNA-222-3p and phosphatase 2 regulatory subunit B alpha were significantly up- and downregulated in activated B cell-like-type diffuse large B-cell lymphoma tissues and cells, respectively. Phosphatase 2 regulatory subunit B alpha was a target of microRNA-222-3p. MicroRNA-222-3p promoted the proliferation and invasion and inhibited the apoptosis of activated B cell-like-type diffuse large B-cell lymphoma cells. Phosphatase 2 regulatory subunit B alpha reversed the tumor-promoting effects of microRNA-222-3p on activated B cell-like-type diffuse large B-cell lymphoma cells. In addition, microRNA-222-3p promoted the tumor growth in mice and downregulated phosphatase 2 regulatory subunit B alpha in tumor tissues.
CONCLUSION
MicroRNA-222-3p promoted the proliferation and invasion and inhibited the apoptosis of activated B cell-like-type diffuse large B-cell lymphoma cells through suppressing phosphatase 2 regulatory subunit B alpha expression.
目的
本研究旨在探讨 microRNA-222-3p 对活化 B 细胞样型弥漫性大 B 细胞淋巴瘤细胞的影响，以及 microRNA-222-3p 与磷酸酶 2 调节亚基 Bα之间的调控关系。
方法
采用实时定量逆转录聚合酶链反应检测活化 B 细胞样型弥漫性大 B 细胞淋巴瘤组织和细胞中 microRNA-222-3p 的表达。通过 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2-H-四唑溴盐（MTT）、集落形成、流式细胞术和 Transwell 实验分析 microRNA-222-3p 对活化 B 细胞样型弥漫性大 B 细胞淋巴瘤细胞增殖、侵袭和凋亡的调控作用。通过荧光素酶报告基因和 RNA 下拉实验确定 microRNA-222-3p 与磷酸酶 2 调节亚基 Bα之间的调控关系。此外，进一步在小鼠中分析了 microRNA-222-3p 对肿瘤生长的影响。
结果
microRNA-222-3p 和磷酸酶 2 调节亚基 Bα 在活化 B 细胞样型弥漫性大 B 细胞淋巴瘤组织和细胞中均显著上调和下调。磷酸酶 2 调节亚基 Bα是 microRNA-222-3p 的靶标。microRNA-222-3p 促进了活化 B 细胞样型弥漫性大 B 细胞淋巴瘤细胞的增殖、侵袭，抑制了其凋亡。磷酸酶 2 调节亚基 Bα逆转了 microRNA-222-3p 对活化 B 细胞样型弥漫性大 B 细胞淋巴瘤细胞的促瘤作用。此外，microRNA-222-3p 促进了小鼠肿瘤的生长，并下调了肿瘤组织中的磷酸酶 2 调节亚基 Bα。
结论
microRNA-222-3p 通过抑制磷酸酶 2 调节亚基 Bα 的表达，促进了活化 B 细胞样型弥漫性大 B 细胞淋巴瘤细胞的增殖、侵袭，抑制了其凋亡。
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