研究来自 Jabalpur 肉鸡的 ESBL 酶中油和 的叶子的抑制潜力和抑制百分比。
Study of inhibitory potential and percent inhibition of oil of and leaves of on ESBL enzyme from in broilers of Jabalpur.
机构信息
Department of Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur, Madhya Pradesh, India.
Department of Microbiology, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur, Madhya Pradesh, India.
出版信息
Indian J Pharmacol. 2019 Sep-Oct;51(5):337-342. doi: 10.4103/ijp.IJP_87_17. Epub 2019 Nov 26.
OBJECTIVE
The inhibitory potential and percent inhibition of oil and fresh juice of leaves on beta-lactamase enzyme of cecal samples of healthy broilers were studied on samples phenotypically positive for extended-spectrum beta-lactamase (ESBL)-producing .
MATERIALS AND METHODS
Four hundred cecal samples screened for ESBL-producing were collected from 38 poultry sale outlets located in Jabalpur. The effect of oil and leaves was seen by colorimetric assay with CENTA and Nitrocefin as chromogenic substrate.
RESULTS
Mean absorbance value was inversely propotional to the inhibitory potential. exhibited 0.4±0.02 and 0.41±0.03 mean absorbance value, 28 per cent and 27 per cent of inhibition with CENTA and Nitrocefin respectively. mean absorbance value and per cent inhibition with CENTA and Nitrocefin was 2.03±0.02 and 10.0 ; 1.97±0.06 and 10.0 respectively (p>0.05) showing non- significant difference in CENTA and Nitrocefin activity. Tazobactum (100 μM) as standard control exhibited a mean absorbance value of 0.12 ± 0.01 and 0.13 ± 0.01 and percent inhibition of 99.88 and 98 against CENTA and Nitrocefin, respectively. Combination of and Syzigium aromaticum showed range of 1.69±0.05 to 1.90±0.08 1.61±0.06 to 1.92±0.08 of absorbance value with per cent inhibition of 14 to 15.9 with CENTA and Nitrocefin respectively.
CONCLUSION
The results depicted that the inhibition of beta-lactamase enzyme activity with oil was higher than that of leaf juice, and combination of both the herbs showed not much difference in activity.
目的
研究油和新鲜叶汁对健康肉鸡盲肠样本中β-内酰胺酶的抑制潜力和抑制百分率，盲肠样本在表型上为产超广谱β-内酰胺酶（ESBL）的 阳性。
材料和方法
从位于 Jabalpur 的 38 个家禽销售点收集了 400 个筛选产 ESBL 的盲肠样本。使用 CENTA 和 Nitrocefin 作为显色底物的比色法来观察 油和 叶的作用。
结果
平均吸光度值与抑制潜力成反比。CENTA 和 Nitrocefin 的 平均吸光度值分别为 0.4±0.02 和 0.41±0.03，抑制率分别为 28%和 27%。CENTA 和 Nitrocefin 的 平均吸光度值和抑制率分别为 2.03±0.02 和 10.0%；1.97±0.06 和 10.0%（p>0.05），CENTA 和 Nitrocefin 活性无显著差异。标准对照物他唑巴坦（100 μM）的平均吸光度值分别为 0.12 ± 0.01 和 0.13 ± 0.01，对 CENTA 和 Nitrocefin 的抑制率分别为 99.88%和 98%。与 Syzigium aromaticum 联合使用，CENTA 和 Nitrocefin 的吸光度值范围分别为 1.69±0.05 至 1.90±0.08，抑制率分别为 14%至 15.9%。
结论
结果表明， 油对β-内酰胺酶活性的抑制作用高于叶汁，两种草药的组合在活性上没有太大差异。
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