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相对底物亲和力指数值：一种鉴定β-内酰胺酶并预测β-内酰胺治疗成功与否的方法。

Relative substrate affinity index values: a method for identification of beta-lactamase enzymes and prediction of successful beta-lactam therapy.

作者信息

James R

出版信息

J Clin Microbiol. 1983 May;17(5):791-8. doi: 10.1128/jcm.17.5.791-798.1983.

DOI:10.1128/jcm.17.5.791-798.1983
PMID:6345576
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272742/
Abstract

Using a nitrocefin competition assay, I determined the relative substrate affinity index (RSAI) values of nine clinically significant beta-lactamase enzymes against a range of beta-lactams. Using selected beta-lactam substrates, I observed large differences in the RSAI values of the nine enzymes that were sufficient in many cases to positively identify specific enzymes. I made use of the unique RSAI values of SHV-1, TEM-1, and TEM-2 beta lactamases with cefoxitin to screen for the presence of these enzymes in Klebsiella aerogenes clinical isolates. The RSAI values also allow for the prediction of the outcome of beta-lactam therapy against specific beta-lactamase-producing isolates.

摘要

通过硝基头孢菌素竞争试验，我测定了9种具有临床意义的β-内酰胺酶对一系列β-内酰胺类药物的相对底物亲和力指数（RSAI）值。使用选定的β-内酰胺类底物，我观察到这9种酶的RSAI值存在很大差异，在许多情况下，这些差异足以明确鉴定特定的酶。我利用头孢西丁对SHV-1、TEM-1和TEM-2β-内酰胺酶的独特RSAI值来筛查产气克雷伯菌临床分离株中这些酶的存在情况。RSAI值还可用于预测针对特定产β-内酰胺酶分离株的β-内酰胺治疗结果。

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本文引用的文献

1
beta-Lactamase proceeds via an acyl-enzyme intermediate. Interaction of the Escherichia coli RTEM enzyme with cefoxitin.β-内酰胺酶通过酰基酶中间体起作用。大肠杆菌RTEM酶与头孢西丁的相互作用。
Biochemistry. 1980 Jun 24;19(13):2895-901. doi: 10.1021/bi00554a012.
2
Enterobacter cloacae outer membrane permeability to ceftizoxime (FK 749) and five other new cephalosporin derivatives.阴沟肠杆菌外膜对头孢唑肟（FK 749）及其他五种新型头孢菌素衍生物的通透性。
J Antibiot (Tokyo). 1980 Mar;33(3):317-21. doi: 10.7164/antibiotics.33.317.
3
Properties of an R factor from Pseudomonas aeruginosa.铜绿假单胞菌R因子的特性
J Bacteriol. 1971 Dec;108(3):1244-9. doi: 10.1128/jb.108.3.1244-1249.1971.
4
Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.一种使用显色头孢菌素底物检测β-内酰胺酶的新方法。
Antimicrob Agents Chemother. 1972 Apr;1(4):283-8. doi: 10.1128/AAC.1.4.283.
5
Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12.参与大肠杆菌K12分裂、伸长和形态形成的不同青霉素结合蛋白。
Proc Natl Acad Sci U S A. 1975 Aug;72(8):2999-3003. doi: 10.1073/pnas.72.8.2999.
6
The beta-lactamases of gram-negative bacteria and their role in resistance to beta-lactam antibiotics.革兰氏阴性菌的β-内酰胺酶及其在对β-内酰胺类抗生素耐药性中的作用。
J Antimicrob Chemother. 1976 Jun;2(2):115-57. doi: 10.1093/jac/2.2.115.
7
Types of beta-lactamase determined by plasmids in gram-negative bacteria.革兰氏阴性菌中由质粒决定的β-内酰胺酶类型。
J Bacteriol. 1979 Jun;138(3):657-62. doi: 10.1128/jb.138.3.657-662.1979.