Mollashahee-Kohkan Fatemeh, Saravani Ramin, Khalili Tahereh, Galavi Hamidreza, Sargazi Saman
Cellular and Molecular Research Center, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Rep Biochem Mol Biol. 2019 Jul;8(2):119-125.
Studies have shown that zinc finger protein 703 (ZNF703) is overexpressed in breast cancer. Levisticum (L.) is a herbal plant with proven medical characteristics in traditional medicine. The purpose of the present study was to evaluate the effect of hydroalcoholic extract of (HELO) on both estrogen receptor-positive (ER) and -negative (ER) cell lines (MCF-7 and MDA-MB-468, respectively).
The anti-proliferative and apoptotic activities of HELO were investigated on both cell lines using MTT and flow-cytometry methods. Real-time PCR was employed to determinate the changes in mRNA expression of the gene.
The 50% maximal inhibitory concentrations (IC) of HELO on ER and ER- cells were 200 and 150 µg/mL after 48 h-treatment. Statistically significant increases in both early and late apoptosis rates were seen in exposed cell lines. expression was less from 4 to 24 h HELO treatment than in untreated cells, and expression was higher in the more invasive MDA-MB-468 cells than in the less invasive MCF-7 cells. Our results demonstrated that HELO induces apoptosis and decreases cell growth in both cell lines.
Our data suggest that HELO alters the mRNA levels of gene while inducing apoptotic cell death in breast cancer-derived cell lines. The use of suppression can be considered as a beneficial target in breast cancer research.
研究表明，锌指蛋白703（ZNF703）在乳腺癌中过表达。欧当归是一种在传统医学中具有经证实的医学特性的草本植物。本研究的目的是评估欧当归水醇提取物（HELO）对雌激素受体阳性（ER）和阴性（ER）细胞系（分别为MCF-7和MDA-MB-468）的影响。
使用MTT和流式细胞术方法研究HELO对两种细胞系的抗增殖和凋亡活性。采用实时PCR测定该基因mRNA表达的变化。
48小时处理后，HELO对ER和ER-细胞的50%最大抑制浓度（IC）分别为200和150μg/mL。在暴露的细胞系中，早期和晚期凋亡率均有统计学显著增加。HELO处理4至24小时后，表达低于未处理细胞，且在侵袭性更强的MDA-MB-468细胞中的表达高于侵袭性较弱的MCF-7细胞。我们的结果表明，HELO在两种细胞系中均诱导凋亡并降低细胞生长。
我们的数据表明，HELO在诱导乳腺癌来源的细胞系凋亡性细胞死亡的同时，改变了该基因的mRNA水平。抑制该基因的表达可被视为乳腺癌研究中的一个有益靶点。
