Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Clin Lab Anal. 2023 May;37(9-10):e24931. doi: 10.1002/jcla.24931. Epub 2023 Jun 20.
Despite the fact that numerous studies have investigated the association between genetic polymorphisms and colorectal cancer (CRC), more research is required to comprehend the molecular mechanisms of CRC. In the present study, we investigated the association between lncRNA HOTAIR rs2366152 and rs1899663 polymorphisms with CRC susceptibility in the Iranian population.
This case-control study consisting of 187 CRC patients and 200 healthy samples. The tetra-amplification refractory mutation system-polymerase chain reaction (Tetra-ARMS-PCR) technique was used for the genotyping of rs2366152 and rs1899663 polymorphisms.
The findings showed that the AG genotype of the rs2366152 polymorphism has a protective effect on CRC susceptibility (OR = 0.60, 95% CI: 0.38-0.94, p-value = 0.023). Furthermore, rs2366152 polymorphism associated with CRC risk in an over dominant inheritance model (p-value = 0.0089). According to the outcomes of the rs1899663 polymorphism, the GT genotype had protective effects on CRC risk (OR = 0.55, 95% CI: 0.35-0.86, p-value = 0.008). Moreover, statistical analysis has shown that the rs1899663 polymorphism was associated with CRC risk in dominant (p-value = 0.013) and overdominant (p-value = 0.0086) inheritance models in the Iranian population.
This study confirmed that HOTAIR rs2366152 and rs1899663 polymorphisms associated with CRC risk in different inheritance models. It is indeed necessary to do additional research to verify our findings.
尽管有大量研究探讨了遗传多态性与结直肠癌（CRC）之间的关系，但仍需要更多的研究来理解 CRC 的分子机制。在本研究中，我们研究了 lncRNA HOTAIR rs2366152 和 rs1899663 多态性与伊朗人群 CRC 易感性的关系。
这项病例对照研究包括 187 例 CRC 患者和 200 例健康对照。采用四扩增不可重复突变系统-聚合酶链反应（Tetra-ARMS-PCR）技术对 rs2366152 和 rs1899663 多态性进行基因分型。
结果表明，rs2366152 多态性的 AG 基因型对 CRC 易感性具有保护作用（OR=0.60，95%CI：0.38-0.94，p 值=0.023）。此外，rs2366152 多态性在超显性遗传模型中与 CRC 风险相关（p 值=0.0089）。根据 rs1899663 多态性的结果，GT 基因型对 CRC 风险具有保护作用（OR=0.55，95%CI：0.35-0.86，p 值=0.008）。此外，统计分析表明，rs1899663 多态性与伊朗人群的显性（p 值=0.013）和超显性（p 值=0.0086）遗传模型中的 CRC 风险相关。
本研究证实，HOTAIR rs2366152 和 rs1899663 多态性与不同遗传模型中的 CRC 风险相关。确实需要进一步的研究来验证我们的发现。
